ISOLATION AND CHARACTERIZATION OF A YEAST CHROMOSOMAL REPLICATOR
ISOLATION AND CHARACTERIZATION OF A YEAST CHROMOSOMAL REPLICATOR
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DOI：
10.1038/282039a0
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发表时间：
1979-01-01
期刊：
影响因子：
64.8
通讯作者：
DAVIS, RW
中科院分区：
文献类型：
--
作者：
STINCHCOMB, DT;STRUHL, K;DAVIS, RW
一个作为染色体复制子的酵母DNA序列ars1（自主复制序列）已经被分离出来。在转化过程中，ar1允许所有共线性DNA的自主复制。复制子可以集成到其他复制单元中，并以多聚体形式发挥作用。850碱基对的ars1元件与其他酵母序列没有可检测的同源性。这种含有复制子的质粒可用于酵母细胞中DNA序列的分离以及染色体DNA复制的研究。
A yeast [Saccharomyces cerevisiae] DNA sequence that behaves as a chromosomal replicator, ars1 (autonomously replicating sequence), has been isolated. On transformation, ars1 allows autonomous replication of all co-linear DNA. The replicator can integrate into other replication units and can function in multimeric form. The 850 base pair ars1 element has no detectable homology to other yeast sequences. Such replicator-containing plasmids can be used for the isolation of DNA sequences in yeast cells as well as for the study of chromosomal DNA replication.