Crystal Engineering of Self-Assembled Porous Protein Materials in Living Cells
Crystal Engineering of Self-Assembled Porous Protein Materials in Living Cells
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DOI：
10.1021/acsnano.6b06099
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发表时间：
2017-03-01
期刊：
影响因子：
17.1
通讯作者：
Ueno, Takafumi
中科院分区：
文献类型：
--
作者：
Abe, Satoshi;Tabe, Hiroyasu;Ueno, Takafumi
晶体多孔材料在分子存储、分离和催化等方面具有重要的应用前景。随着人们对蛋白质晶体的了解越来越深入，它们的潜力也越来越大。蛋白质晶体被认为是多孔材料，因为它们呈现出高度有序的蛋白质分子三维排列，具有高孔隙率和宽孔径。然而，在活细胞中实现蛋白质晶体的功能化仍然很困难。在这里，我们报道了多面体（polyhedrin单体，PhM）的天然结晶蛋白组装体，可以通过删除位于PhM分子间接触区域的氨基酸残基来扩展多孔网络。在体外和体内条件下，突变型多面体晶体（WTPhC）相对于野生型多面体晶体（WTPhC）的荧光染料的吸附速率和数量都有所增加。这些结果为设计具有分子识别和外源物质在活细胞中的储存应用的自组装蛋白质材料提供了策略，并为生物正交化学和体内晶体结构分析的发展提供了切入点。
Crystalline porous materials have been investigated for development of important applications in molecular storage, separations, and catalysis. The potential of protein crystals is increasing as they become better understood. Protein crystals have been regarded as porous materials because they present highly ordered 3D arrangements of protein molecules with high porosity and wide range of pore sizes. However, it remains difficult to functionalize protein crystals in living cells. Here, we report that polyhedra, a natural crystalline protein assembly of polyhedrin monomer (PhM) produced in insect cells infected by cypovirus, can be engineered to extend porous networks by deleting selected amino acid residues located on the intermolecular contact region of PhM. The adsorption rates and quantities of fluorescent dyes stored within the mutant crystals are increased relative to those of the wild-type polyhedra crystal (WTPhC) under both in vitro and in vivo conditions. These results provide a strategy for designing self-assembled protein materials with applications in molecular recognition and storage of exogenous substances in living cell as well as an entry point for development of bioorthogonal chemistry and in vivo crystal structure analysis.