Liver Tumor Spheroid Reconstitution for Testing Mitochondrial Targeted Magnetic Hyperthermia Treatment
Liver Tumor Spheroid Reconstitution for Testing Mitochondrial Targeted Magnetic Hyperthermia Treatment
复制标题
肝肿瘤球体重建用于测试线粒体靶向磁热疗
DOI：
10.1021/acsbiomaterials.8b01630
复制
发表时间：
2019
影响因子：
--
通讯作者：
Fan Haiming
中科院分区：
文献类型：
--
作者：
Peng Xuqi;Wang Bingquan;Yang Yu;Zhang Yihan;Liu Yonggang;He Yuan;Zhang Ce;Fan Haiming
线粒体靶向纳米治疗因其能够通过外部磁和光刺激破坏线粒体功能并诱导肿瘤细胞凋亡而受到广泛关注。然而，当应用于动物模型时，效果显著减弱。关键因素包括体内环境的复杂性和肿瘤组织的内在保护特性。为了解决这些障碍，并减少药物筛选的费用，我们在此介绍了一种方法，用于生产毫米大小的球体的结构和功能特性的肿瘤组织在体内。通过比较TPP-SPION（三苯基膦阳离子-超顺磁性氧化铁纳米颗粒）对单层培养的HepG 2细胞和球体的作用，证明球体作为肝肿瘤模型的必要性。我们的研究表明，与单层细胞相比，大规模的球体更能反映体内肿瘤的特征，并且在磁热疗过程中对TPP-SPION的反应性较低。
Mitochondria-targeting nanotherapy receives great attention these days for its capacity in disrupting mitochondria function and inducing tumor cell apoptosis through external magnetic and optical stimulations. However, the effect is significantly diminished when applied to animal models. The key factors include environmental complexity in vivo and intrinsic protective features of tumor tissues. To address these obstacles and reduce expenses on drug screening, we herein introduce a methodology for producing millimeter-sized spheroids with structural and functional characteristics of tumor tissues in vivo. The necessity of spheroid as a liver tumor model is demonstrated by comparing the effect of TPP-SPIONs (triphenylphosphonium cation-superparamagnetic iron oxide nanoparticles) on monolayer-cultured HepG2 cells and spheroids. Our study reveals that large-scale spheroid, in contrast to monolayer cells, reflects more in vivo tumor characters and is less responsive to TPP-SPIONs during magnetic hyperthermia treatment.