Activity-based protein profiling for biochemical pathway discovery in cancer.
Activity-based protein profiling for biochemical pathway discovery in cancer.
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DOI：
10.1038/nrc2901
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发表时间：
2010-09
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大规模的分析方法已经发现了许多与肿瘤发生相关的基因和蛋白质表达变化。然而，确定这些表达变化的相关性以及它们影响哪些生化途径一直受到我们对蛋白质组及其无数功能和调控模式的不完全理解的阻碍。基于活性的分析平台能够发现癌症相关酶和选择性药理学探针，以干扰和表征肿瘤细胞中的这些蛋白质。当与其他大规模分析方法相结合时，基于活性的蛋白质组学可以深入了解支持癌症发病机制的代谢和信号通路，并阐明疾病诊断和治疗的新策略。
Large-scale profiling methods have uncovered numerous gene and protein expression changes that correlate with tumorigenesis. However, determining the relevance of these expression changes and which biochemical pathways they affect has been hindered by our incomplete understanding of the proteome and its myriad functions and modes of regulation. Activity-based profiling platforms enable both the discovery of cancer-relevant enzymes and selective pharmacological probes to perturb and characterize these proteins in tumour cells. When integrated with other large-scale profiling methods, activity-based proteomics can provide insight into the metabolic and signalling pathways that support cancer pathogenesis and illuminate new strategies for disease diagnosis and treatment.