Effects of "loop" diuretics on ion transport by dog tracheal epithelium.
Effects of "loop" diuretics on ion transport by dog tracheal epithelium.
复制标题
“袢”利尿剂对狗气管上皮离子转运的影响。
DOI：
10.1152/ajpcell.1983.245.5.c388
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Highland,E
中科院分区：
文献类型：
--
作者：
Widdicombe,JH;Nathanson,IT;Highland,E
“袢”利尿剂MK-196、布美他尼、哌瑞他尼和呋塞米均为犬气管上皮Cl转运的强效抑制剂。在短路的组织中，药物引起的单向氯通量和氯向管腔的净通量显着减少;净氯通量的变化与短路电流的变化无显着差异。药物对活性钠吸收无影响。所有药物均导致组织电导显著下降。除MK-196外，所有药物在血清浴中的效力更强; MK-196在组织两侧的效力相等。在离体细胞实验中，利尿剂未引起细胞内Na和K浓度的显著变化，细胞内Cl浓度下降，Na和Cl流入量大致相等福尔斯。这些结果表明，这些药物的作用部位是在基底外侧连接的钠-氯进入过程。这种关联的进入过程的其他证据提供了实验中，去除氯减少钠流入和去除钠减少氯流入。
The "loop" diuretics MK-196, bumetanide, piretanide, and furosemide are all potent inhibitors of Cl transport by the dog's tracheal epithelium. In short-circuited tissues, the drugs caused significant decreases in both unidirectional Cl fluxes and in the net flux of Cl toward the lumen; the change in net Cl flux was not significantly different from the change in short-circuit current. The drugs had no effect on active Na absorption. All drugs caused a significant fall in tissue conductance. All drugs, except MK-196, were more potent from the serosal bath; MK-196 was equipotent from either side of the tissue. In experiments with isolated cells, the diuretics caused no significant changes in intracellular Na and K concentrations, a fall in intracellular Cl concentration, and approximately equal falls in Na and Cl influxes. These results suggest that the site of action of these drugs is on a basolateral linked Na-Cl entry process. Additional evidence for such a linked entry process was provided by experiments in which removal of Cl reduced Na influx and removal of Na reduced Cl influx.