ULTRAVIOLET ACTION SPECTRUM FOR FLUOROGEN PRODUCTION IN THE OCULAR LENS
ULTRAVIOLET ACTION SPECTRUM FOR FLUOROGEN PRODUCTION IN THE OCULAR LENS
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眼晶状体中产生荧光的紫外线作用光谱
DOI：
10.1111/j.1751-1097.1977.tb07463.x
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发表时间：
1977
影响因子：
3.3
通讯作者：
S. Lerman
中科院分区：
文献类型：
--
作者：
R. Borkman;A. Dalrymple;S. Lerman
摘要——……之前的工作表明，荧光物质（360nm 激发，440nm 发射）的浓度通常随着人类晶状体年龄的增长而增加，可以通过将培养的人类或动物晶状体暴露在紫外线辐射下人工产生。在本文中，我们报告了体外大鼠晶状体中这种荧光材料的产生速率与紫外线照射波长的关系的测量结果。观察到的荧光产生速率标准化为恒定光子通量与照射波长的关系图显示，在 360 或 320 nm 处几乎没有作用，在 300 nm 处急剧增加，在 300–280 nm 范围内保持相对恒定，然后从 270–250 nm 进一步逐渐上升。将大鼠晶状体的结果与其他地方报道的水溶液中色氨酸的结果进行比较。溶液中色氨酸光化学破坏的作用光谱与大鼠晶状体中荧光产生的作用光谱非常相似。这一结果和其他证据表明，色氨酸的光化学破坏可能是紫外线诱导眼晶状体产生荧光剂的初始事件。
Abstract— …Previous work has demonstrated that fluorescent material (360nm excitation, 440nm emission), whose concentration normally increases with age in human lenses, can be generated artificially by exposing cultured human or animal lenses to UV radiation. In the present paper we report measurements of the rate of production of this fluorescent material in rat lenses in vitro as a function of UV irradiation wavelength. A plot of the observed rate of fluorogen production normalized to constant photon flux vs irradiation wavelength shows little action at 360 or 320nm, increases sharply at 300nm, remains relatively constant in the range 300–280nm, and then exhibits a further gradual rise from 270–250nm. The results on rat lenses are compared with results reported elsewhere for tryptophan in aqueous solution. The action spectrum for photochemical destruction of tryptophan in solution closely parallels that for fluorogen production in rat lenses. This result and other evidence suggest that photochemical destruction of tryptophan might be the initial event in UV‐induced fluorogen production in the ocular lens.