Effects of fish collagen peptides on collagen post-translational modifications and mineralization in an osteoblastic cell culture system.
Effects of fish collagen peptides on collagen post-translational modifications and mineralization in an osteoblastic cell culture system.
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DOI：
10.4012/dmj.2012-220
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发表时间：
2013
影响因子：
2.5
通讯作者：
Yamauchi M
中科院分区：
文献类型：
--
作者：
Yamada S;Nagaoka H;Terajima M;Tsuda N;Hayashi Y;Yamauchi M
胶原蛋白是组织工程和再生医学中应用最广泛的生物材料之一。鱼胶原蛋白肽（FCP）已被用作膳食补充剂，但其对细胞功能的影响仍知之甚少。本研究的目的是利用成骨细胞 MC3T3-E1 细胞培养系统研究 FCP 对胶原蛋白合成、质量和矿化的影响。用 FCP 处理的细胞显着上调了几种胶原蛋白修饰酶的基因表达，并且更多的胶原蛋白沉积在培养物中。与未处理组相比，处理组中的胶原蛋白表现出更大程度的赖氨酸羟基化、更高水平的羟基赖氨酸-醛衍生交联以及加速的交联成熟。此外，治疗组显示出加速的基质矿化。这些结果表明，FCP 在胶原合成、质量和矿化方面对成骨细胞产生积极影响，从而表明 FCP 在骨组织工程中的潜在用途。
Collagen is one of the most widely used biomaterials for tissue engineering and regenerative medicine. Fish collagen peptides (FCP) have been used as a dietary supplement, but their effects on the cellular function are still poorly understood. The objective of this study was to investigate the effects of FCP on collagen synthesis, quality and mineralization using an osteoblastic MC3T3-E1 cell culture system. Cells treated with FCP significantly upregulated the gene expression of several collagen modifying enzymes and more collagen was deposited in the cultures. Collagen in the treated group showed a greater extent of lysine hydroxylation, higher levels of hydroxylysine-aldehyde derived cross-links and accelerated cross-link maturation compared with the untreated group. Furthermore, the treated group showed accelerated matrix mineralization. These results indicate that FCP exerts a positive effect on osteoblastic cells in terms of collagen synthesis, quality and mineralization, thereby suggesting the potential utility of FCP for bone tissue engineering.
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影响因子：
1.6
作者：
Tanaka, Midori;Koyama, Yoh-ichi;Nomura, Yoshihiro
通讯作者：
Nomura, Yoshihiro
影响因子：
4.2
作者：
Turecek, C.;Fratzl-Zelman, N.;Varga, F.
通讯作者：
Varga, F.
影响因子：
5.3
作者：
Schegg, Belinda;Huelsmeier, Andreas J.;Hennet, Thierry
通讯作者：
Hennet, Thierry
影响因子：
5.6
作者：
Hong, HH;Pischon, N;Trackman, PC
通讯作者：
Trackman, PC
影响因子：
6.2
作者：
Pornprasertsuk, S;Duarte, WR;Yamauchi, M
通讯作者：
Yamauchi, M