Preparation and Assembly of Poly (ar ginine)-coate d liposomes to Create a Free-standing Bio-scaffold
Preparation and Assembly of Poly (ar ginine)-coate d liposomes to Create a Free-standing Bio-scaffold
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聚精氨酸包被脂质体的制备和组装以创建独立式生物支架
DOI：
10.1021/la201500b
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发表时间：
2011
期刊：
影响因子：
3.9
通讯作者：
Keiji Fujimoto
中科院分区：
文献类型：
--
作者：
Saika Yamamoto;Yuuka Fukui;Sachiko Kaihara;Keiji Fujimoto
我们通过聚合物包覆的脂质体的组装创建了一个独立的膜作为一种新型的生物支架。使用具有细胞穿透活性的聚精氨酸（PArg）在带负电荷的脂质体（lipo-PArg）上形成聚合物层。PArg脂质体的囊壁使其能够改善囊的机械性能，并通过静电吸引将脱氧核糖核酸（DNA）展示在囊泡表面（lipo-PArg-DNA）。脂质体-PArg和脂质体-PArg-DNA包封的荧光探针的释放速率可通过囊壁的聚合物层的数量来调节。为了研究lipo-PArg-DNA的细胞膜渗透性，将聚合物包被的脂质体与人脐静脉内皮细胞（HUVEC）在4 °C下孵育。结果发现，脂质体-PAg进行了显着的细胞摄取，而裸脂质体和壳聚糖修饰的脂质体不能克服质膜屏障。为了制备由聚合物包被的脂质体组成的独立膜，将lipo-PArg-DNA的悬浮液浇铸在网孔上并干燥。扫描电子显微镜观察表明，通过干燥介导的组装过程获得了独立的膜，而膜内的聚合物涂层脂质体没有破裂。另一方面，不可能从脂质体-PArgand DNA的混合物中获得完整的膜。总之，脂质体-PArg-DNA胶囊具有作为药物载体的多功能，并且它们的组装使我们能够创建可用作生物支架的独立膜。
We created a free-standing membrane as a novel bioscaffold through the assembly of polymer-coated liposomes. Polyarginine (PArg) possessing a cell-penetrating activity was used to form the polymer layer onto a negatively charged liposome (lipo-PArg). The capsule wall of PArgover liposomes made it possible to improve the mechanical property of capsules and to display deoxyribonucleic acid (DNA) over the vesicle surface through the electrostatic attraction (lipo-PArg–DNA). The release rates of a fluorescent probe encapsulated in lipo-PArgand lipo-PArg–DNA were tunable by the number of polymeric layers of the capsule walls. To investigate the cell-membrane permeability of lipo-PArg–DNA, polymer-coated liposomes were incubated with human umbilical vein endothelial cells (HUVECs) at 4 °C. It was found that lipo-PArgunderwent a significant cellular uptake, whereas bare liposomes and liposomes modified with chitosan were incapable of overcoming the plasma membrane barrier. To prepare a free-standing membrane composed of polymer-coated liposomes, a suspension of lipo-PArg-DNA was cast over a mesh hole and dried up. SEM observation revealed that a free-standing membrane was obtained through drying-mediated assembly process without rupturing polymer-coated liposomes inside the membrane. On the other hand, it was not possible to obtain a complete membrane from a mixture of lipo-PArgand DNA. In summary, lipo-PArg–DNA capsules possess versatile functions as a drug carrier, and their assembly enables us to create a free-standing membrane applicable as a bioscaffold.