A CDNA CLONE CLOSELY ASSOCIATED WITH NON-A-HEPATITIS, NON-B-HEPATITIS
A CDNA CLONE CLOSELY ASSOCIATED WITH NON-A-HEPATITIS, NON-B-HEPATITIS
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DOI：
10.1093/nar/18.9.2685
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发表时间：
1990-05-11
影响因子：
14.9
通讯作者：
SHIKATA, T
中科院分区：
文献类型：
--
作者：
MAENO, M;KAMINAKA, K;SHIKATA, T
lambda gt 11 cDNA 文库是用从具有高丙氨酸转氨酶活性的乙型肝炎病毒表面抗原阴性人血浆中纯化的 RNA 构建的。通过使用来自非甲非乙型肝炎 (NANBH) 携带者和恢复期黑猩猩的混合血清进行免疫筛选，分离出 cDNA 克隆，命名为 C8-2。 C8-2产生的重组蛋白与NANBH慢性期患者的血清发生特异性反应。 C8-2的序列为269 bp，不与任何人类或黑猩猩的基因组DNA杂交，与灵长类动物和病毒的基因组DNA没有同源性。通过RNA印迹杂交和聚合酶链式反应扩增产物的Southern印迹分析表明该序列存在于可能具有传染性的血浆的RNA中。这些结果强烈表明C8-2源自这种病毒性肝炎的病原体。
A lambda gt 11 cDNA library was constructed from RNA purified from hepatitis B viral surface antigen-negative human plasma with high alanine aminotransferase activity. A cDNA clone, designated as C8-2, was isolated by immunoscreening with mixed sera from non-A, non-B hepatitis (NANBH) carrier and convalescent chimpanzees. The recombinant protein produced by C8-2 reacted specifically with sera of patients in the chronic phase of NANBH. The sequence of C8-2, 269 bp, did not hybridize with any human or chimpanzee genomic DNA, had no homology with those of primates and viruses. The existence of this sequence in RNA of possibly infectious plasma was shown by RNA blot hybridization and by Southern blot analysis of products amplified by the polymerase chain reaction. These results strongly suggest that C8-2 is derived from the agent of this viral hepatitis.