Three-dimensional (3-D) imaging of chondrocytes in articular cartilage: Growth-associated changes in cell organization
Three-dimensional (3-D) imaging of chondrocytes in articular cartilage: Growth-associated changes in cell organization
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DOI：
10.1016/j.biomaterials.2006.08.053
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发表时间：
2007-01-01
期刊：
影响因子：
14
通讯作者：
Sah, Robert L.
中科院分区：
文献类型：
--
作者：
Jadin, Kyle D.;Bae, Won C.;Sah, Robert L.
三维（3-D）成像和分析技术可用于评估生物组织中细胞的组织，为细胞排列在生长，稳态和变性中的作用提供关键见解。本研究的目的是使用这种方法来评估生长相关的变化，从不同部位的牛膝关节软骨的细胞组织。获得胎儿、小牛和成人软骨的三维图像并进行处理以识别细胞核。细胞密度随着生长和距关节面深度的增加而降低。通过与最近的相邻细胞的角度评估的细胞组织也随生长而变化，并且反映了成体组织中细胞的经典组织，相邻细胞水平排列在浅区（平均角度为20度）中，垂直排列在深区（60度）中。在软骨的所有其他区域和生长阶段中，角度类似于32度，指示各向同性组织。与此相反，最近邻距离并没有随着生长或深度显着变化。总之，这些结果表明软骨生长与软骨细胞组的独特3-D排列相关。(c)2006爱思唯尔有限公司保留所有权利。
Three-dimensional (3-D) imaging and analysis techniques can be used to assess the organization of cells in biological tissues, providing key insights into the role of cell arrangement in growth, homeostasis, and degeneration. The objective of the present study was to use such methods to assess the growth-related changes in cell organization of articular cartilage from different sites in the bovine knee. Three-dimensional images of fetal, calf, and adult cartilage were obtained and processed to identify cell nuclei. The density of cells was lower with growth and with increasing depth from the articular surface. The cell organization, assessed by the angle to the nearest neighboring cell, also varied with growth, and reflected the classical organization of cells in adult tissue, with neighboring cells arranged horizontally in the superficial zone (average angle of 20 degrees) and vertically in the deep zone (60 degrees). In all other regions and growth stages of cartilage, the angle was similar to 32 degrees, indicative of an isotropic organization. On the contrary, the nearest neighbor distance did not vary significantly with growth or depth. Together, these results indicate that cartilage growth is associated with distinctive 3-D arrangements of groups of chondrocytes. (c) 2006 Elsevier Ltd. All rights reserved.