TGNap1 is required for microtubule-dependent homeostasis of a subpopulation of the plant trans-Golgi network
TGNap1 is required for microtubule-dependent homeostasis of a subpopulation of the plant trans-Golgi network
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DOI：
10.1038/s41467-018-07662-4
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发表时间：
2018-12
影响因子：
16.6
通讯作者：
L. Renna;Giovanni Stefano;Erin Slabaugh;Clarissa Wormsbaecher;A. Sulpizio;K. Zienkiewicz;F. Brandizzi
中科院分区：
文献类型：
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作者：
L. Renna;Giovanni Stefano;Erin Slabaugh;Clarissa Wormsbaecher;A. Sulpizio;K. Zienkiewicz;F. Brandizzi
确定会聚和发散机制的生物起源和功能的内膜细胞器是根本重要的细胞生物学。在所有的真核生物中，高尔基体网络（TGN）是胞吐和胞吞途径交汇的枢纽。为了了解TGN生物发生和功能的机制，我们表征了TGNap1，一种由后生动物保守的未知功能的植物基因编码的蛋白质。我们证明，TGNap1是一种TGN蛋白所需的生物合成和内吞交通途径的稳态。我们还表明，TGNap 1结合Rab6，YIP 4和微管。最后，我们确定TGNap1有助于微管依赖的生物发生，跟踪和功能的TGN子集，可能通过与Rab6和YIP4的相互作用。我们的研究结果确定了一个重要的交通决定因素在植物TGN和揭示了一个意想不到的依赖后高尔基交通稳态和细胞器的生物发生在植物微管。
Defining convergent and divergent mechanisms underlying the biogenesis and function of endomembrane organelles is fundamentally important in cell biology. In all eukaryotes, theTrans-Golgi Network (TGN) is the hub where the exocytic and endocytic pathways converge. To gain knowledge in the mechanisms underlying TGN biogenesis and function, we characterized TGNap1, a protein encoded by a plant gene of unknown function conserved with metazoans. We demonstrate that TGNap1 is a TGN protein required for the homeostasis of biosynthetic and endocytic traffic pathways. We also show that TGNap1 binds Rab6, YIP4 and microtubules. Finally, we establish that TGNap1 contributes to microtubule-dependent biogenesis, tracking and function of a TGN subset, likely through interaction with Rab6 and YIP4. Our results identify an important trafficking determinant at the plant TGN and reveal an unexpected reliance of post-Golgi traffic homeostasis and organelle biogenesis on microtubules in plants.