Quantitative multiplex CARS spectroscopy in congested spectral regions
Quantitative multiplex CARS spectroscopy in congested spectral regions
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DOI：
10.1021/jp0564849
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发表时间：
2006-03-09
影响因子：
3.3
通讯作者：
M端ller, M
中科院分区：
文献类型：
--
作者：
Rinia, HA;Bonn, M;M端ller, M
一种新的程序开发来描述和再现实验相干反斯托克斯拉曼散射（汽车）数据，特别强调高度拥挤的光谱区域。该方法，举例说明了高质量的多重汽车数据，利用自发拉曼散射的结果。结果表明，潜在的振动拉曼响应可以检索从多重汽车光谱，使拉曼光谱可以重建，提供了足够的信号噪声比（SNR）是存在于实验数据和足够的先验知识的振动共振存在。汽车到拉曼数据的转换允许汽车光谱的定量解释。这种新的方法被证明是高度拥挤的多重汽车光谱的腺苷单，二，和三磷酸（AMP，ADP和ATP），烟酰胺腺嘌呤二核苷酸（NAD+），和小单层囊泡（SUV）的1-棕榈酰-2-油酰-sn-甘油-3-磷酸胆碱（POPC）。定量测定的核苷酸浓度和混合物中的组成分析证明。
A novel procedure is developed to describe and reproduce experimental coherent anti-Stokes Raman scattering (CARS) data, with particular emphasis on highly congested spectral regions. The approach, exemplified here with high-quality multiplex CARS data, makes use of spontaneous Raman scattering results. It is shown that the underlying vibrational Raman response can be retrieved from the multiplex CARS spectra, so that the Raman spectrum can be reconstituted, provided an adequate signal-to-noise ratio (SNR) is present in the experimental data and sufficient a priori knowledge of the vibrational resonances involved exists. The conversion of CARS to Raman data permits a quantitative interpretation of CARS spectra. This novel approach is demonstrated for highly congested multiplex CARS spectra of adenosine mono-, di-, and triphosphate (AMP, ADP, and ATP), nicotinamide adenine dinucleotide (NAD+), and small unilamellar vesicles (SUVs) of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC). Quantitative determination of nucleotide concentrations and composition analysis in mixtures is demonstrated.