Vascularization and cellular isolation potential of a novel electrospun cell delivery vehicle.
Vascularization and cellular isolation potential of a novel electrospun cell delivery vehicle.
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新型电纺细胞输送载体的血管化和细胞分离潜力。
DOI：
10.1002/jbm.a.34900
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Williams,StuartK
中科院分区：
文献类型：
--
作者：
Krishnan,Laxminarayanan;Touroo,Jeremy;Reed,Robert;Boland,Eugene;Hoying,JamesB;Williams,StuartK
临床上需要一种细胞递送装置，该装置支持长期细胞活力、装置内的细胞保留以及在必要时回收递送的细胞。以前，细胞分离装置基于中空纤维膜、多孔聚合物支架、藻酸盐系统或微机械加工膜。我们提出了一种新的双孔隙度电纺膜的设备，支持其外部多孔层的细胞浸润和血管化，并保持由内部低孔隙率层界定的管腔内的细胞隔离的发展和表征。静电纺丝条件最初被建立以支持静电纺丝纤维沉积到非导电有机硅表面上。在这些参数的基础上，使用尼龙作为非导电支架来沉积双孔隙度电纺纤维，从而生产出用于体内评价的装置。外部多孔层支持渗透性微循环的发展，并且膜支持胰岛素从包封的持续释放小丸转移4周。植入2周后，可识别植入器械内的活细胞。通过成功证明植入器械内人上皮细胞的存活和细胞分离以及使用该器械输送胰岛素的能力，我们已经确定了该器械在局部细胞移植中的实用性。细胞递送装置建立了一个平台，以测试在修饰的自体或同种异体细胞的临床使用中在植入部位的细胞剂量控制和细胞定位的方法的可行性。© 2013威利期刊公司. J Biomed Mater Res Part A：102A：2208-2219，2014.
A clinical need exists for a cell delivery device that supports long‐term cell viability, cell retention within the device and retrieval of delivered cells if necessary. Previously, cell isolation devices have been based on hollow fiber membranes, porous polymer scaffolds, alginate systems, or micro‐machined membranes. We present the development and characterization of a novel dual porosity electrospun membrane based device, which supports cellular infiltration and vascularization of its outer porous layer and maintains cellular isolation within a lumen bounded by an inner low porosity layer. Electrospinning conditions were initially established to support electrospun fiber deposition onto nonconductive silicone surfaces. With these parameters established, devices forin vivoevaluations were produced using nylon as a nonconductive scaffold for deposition of dual porosity electrospun fibers. The outer porous layer supported the development of a penetrating microcirculation and the membrane supported the transfer of insulin from encapsulated sustained release pellets for 4 weeks. Viable cells implanted within the device could be identified after 2 weeks of implantation. Through the successful demonstration of survival and cellular isolation of human epithelial cells within the implanted devices and the ability to use the device to deliver insulin, we have established the utility of this device toward localized cell transplantation. The cell delivery device establishes a platform to test the feasibility of approaches to cell dose control and cell localization at the site of implantation in the clinical use of modified autologous or allogeneic cells. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 2208–2219, 2014.
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经心肌激光血运重建：组织病理学发现。
DOI：
10.1016/s1054-8807(97)00061-6
发表时间：
1998
期刊：
Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology
影响因子：
--
作者：
K. Dědič;Tomas Klima;D. A. Cooley;O. Frazier;K. Kadipasaoglu;Hasan B. Cihan
通讯作者：
Hasan B. Cihan
DOI：
10.1557/proc-530-7
发表时间：
1998
期刊：
MRS Proceedings
影响因子：
--
作者：
T. Desai;D. Hansford;W. Chu;T. Huen;M. Ferrari
通讯作者：
M. Ferrari
免疫隔离膜的新生血管形成：膜结构和包膜组织的影响。
影响因子：
0.9
作者：
J. Brauker;L. Martinson;R. Hill;S. Young;V. Carr;R. C. Johnson
通讯作者：
R. C. Johnson
影响因子：
2.8
作者：
Desai, Tejal A.;Chu, Wen Hwa;Ferrari, Mauro
通讯作者：
Ferrari, Mauro
影响因子：
--
作者：
Griffith, CK;Miller, C;George, SC
通讯作者：
George, SC