Chronic menthol attenuates the effect of nicotine on body temperature in adolescent rats
Chronic menthol attenuates the effect of nicotine on body temperature in adolescent rats
复制标题
DOI：
10.1080/14622200802443734
复制
发表时间：
2008-01-01
影响因子：
4.7
通讯作者：
LaHoste, Gerald J.
中科院分区：
文献类型：
--
作者：
Ruskin, David N.;Anand, Rene;LaHoste, Gerald J.
薄荷醇是烟草制品中常用的添加剂。对于薄荷卷烟吸烟者，尤其是青少年吸烟者来说，戒烟可能更加困难。有证据表明薄荷醇可以影响神经递质受体和尼古丁代谢。我们使用体温作为体内急性尼古丁影响的生物测定来研究慢性薄荷醇的影响。雄性大鼠（34-36天，青少年；53-58天，青年；9-10个月，成年）每天注射一次薄荷醇（100mg/kg）或媒介物，持续4天。第5天，给动物注射尼古丁（0.5mg/kg）并在接下来的70分钟内测量体温。我们发现长期薄荷醇治疗或年龄对基线温度没有影响。尼古丁很快就会使所有动物的血管舒张而导致体温降低。长期薄荷醇治疗仅对青春期大鼠有显着效果，可减少尼古丁引起的体温过低。第 6 天重复尼古丁治疗以测试耐受性。在所有年龄段中都发现了同等的耐受性，薄荷醇的减弱作用仍然存在，并且仍然仅限于青春期大鼠。在青少年中，在尼古丁前30分钟注射急性薄荷醇（400mg/kg）也可以减轻尼古丁引起的体温过低，但效果较小。同样在青少年中，我们发现慢性或急性薄荷醇对肼屈嗪（一种外周作用血管扩张剂）引起的体温过低没有影响。这些数据表明，薄荷醇减少了尼古丁对青少年体温的影响，表明青少年生理对薄荷醇的敏感性更高。
Menthol is a commonly used additive in tobacco products. Smoking cessation may be more difficult for smokers of mentholated cigarettes, particularly adolescent smokers. Evidence indicates that menthol can influence neurotransmitter receptors and nicotine metabolism. We investigated the effects of chronic menthol using body temperature as a bioassay for the effects of acute nicotine in vivo. Male rats (34-36 days, adolescent; 53-58 days, young adult; 9-10 months, full adult) were injected with menthol (100mg/kg) or vehicle once daily for 4 days. On day 5, animals were injected with nicotine (0.5mg/kg) and body temperature was measured for the next 70min. We found no effect of chronic menthol treatment or of age on baseline temperature. Nicotine quickly produced vasodilatory hypothermia in all animals. Chronic menthol treatment had significant effects only in adolescent rats, diminishing nicotine-induced hypothermia. Nicotine treatment was repeated on day 6 to test for tolerance. Equivalent tolerance was found in all ages, and the attenuating effect of menthol was still present and was still limited to adolescent rats. In adolescents, acute menthol injection (400mg/kg) 30min prior to nicotine also attenuated nicotine-induced hypothermia but with a smaller effect size. Also in adolescents, we found no effect of chronic or acute menthol on hypothermia induced by hydralazine, a peripherally acting vasodilator. These data demonstrate that menthol diminishes the influence of nicotine on body temperature in adolescents, suggesting a greater susceptibility of youthful physiology to menthol.