TaMCA1, a regulator of cell death, is important for the interaction between wheat and Puccinia striiformis.
TaMCA1, a regulator of cell death, is important for the interaction between wheat and Puccinia striiformis.
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TaMCA1 是细胞死亡的调节因子，对于小麦和条锈菌之间的相互作用非常重要
DOI：
10.1038/srep26946
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发表时间：
2016-05-27
影响因子：
4.6
通讯作者：
Kang Z
中科院分区：
文献类型：
--
作者：
Hao Y;Wang X;Wang K;Li H;Duan X;Tang C;Kang Z
Metacaspase同源基因在真菌、原生动物和植物中都是保守的，然而，它们在植物抗病中的作用在很大程度上是未知的。在本研究中，我们发现了一个小麦偏天冬酶基因TaMCA1，该基因有三个拷贝，分别位于1A、1B和1D染色体上。TaMCA1蛋白含有典型的I型中位天冬氨酸结构域的结构特征，其中包括一个N末端的前结构域。瞬时表达分析表明，TaMCA1定位于胞浆和线粒体。TaMCA1在体外不显示caspase-1活性，但能抑制小鼠bax基因诱导的烟草和小麦叶片细胞死亡。此外，条锈菌攻击后，TaMCA1的表达水平也上调。小麦(PST)。通过病毒诱导的基因沉默(VIGS)敲除TaMCA1可以增强植物对PST的抗病能力，并增加过氧化氢(H_2O_2)的积累。进一步的研究表明，TaMCA1降低了酵母细胞的抗性，其作用类似于酵母Metacaspase，并且TaMCA1和TaLSD1之间没有相互作用。根据这些综合结果，我们推测TaMCA1，一个细胞死亡的调节因子，在小麦和PST的亲和相互作用中起着重要的作用。
Metacaspase orthologs are conserved in fungi, protozoa and plants, however, their roles in plant disease resistance are largely unknown. In this study, we identified a Triticum aestivum metacaspase gene, TaMCA1, with three copies located on chromosomes 1A, 1B and 1D. The TaMCA1 protein contained typical structural features of type I metacaspases domains, including an N-terminal pro-domain. Transient expression analyses indicated that TaMCA1 was localized in cytosol and mitochondria. TaMCA1 exhibited no caspase-1 activity in vitro, but was able to inhibit cell death in tobacco and wheat leaves induced by the mouse Bax gene. In addition, the expression level of TaMCA1 was up-regulated following challenge with the Puccinia striiformis f. sp. tritici (Pst). Knockdown of TaMCA1 via virus-induced gene silencing (VIGS) enhanced plant disease resistance to Pst, and the accumulation of hydrogen peroxide (H2O2). Further study showed that TaMCA1 decreased yeast cell resistance similar to the function of yeast metacaspase, and there was no interaction between TaMCA1 and TaLSD1. Based on these combined results, we speculate that TaMCA1, a regulator of cell death, is important during the compatible interaction of wheat and Pst.