Fusaric acid induces apoptosis in saffron root-tip cells: roles of caspase-like activity, cytochrome c, and H2O2
Fusaric acid induces apoptosis in saffron root-tip cells: roles of caspase-like activity, cytochrome c, and H2O2
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DOI：
10.1007/s00425-006-0345-6
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发表时间：
2006-07
期刊：
影响因子：
4.3
通讯作者：
L. Samadi;B. Shahsavan Behboodi
中科院分区：
文献类型：
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作者：
L. Samadi;B. Shahsavan Behboodi
程序性细胞死亡（PCD），现在称为凋亡，伴随着特定的形态学特征。在这项研究中，镰刀菌酸，afusariummycotoxin，被用来检查细胞死亡的番红花（Crocus sativusLinnaeus）根，使用几种凋亡测定。我们的研究结果表明，中等剂量的FA（50-100 μM）诱导凋亡特征，而高剂量的FA（> 200 μM）刺激坏死。中等剂量的FA诱导的凋亡样特征包括染色质凝聚、从核出芽的凝聚染色质球的形成、核小体DNA断裂成约180 bp的片段、磷脂酰丝氨酸暴露于外膜小叶、细胞色素递送至胞质溶胶以及H2 O2的产生。在丝氨酸蛋白酶、半胱天冬酶-1或半胱天冬酶-3抑制剂的存在下，未观察到根细胞中的这些凋亡改变。有人提出，H2 O2的产生和细胞色素释放到细胞溶胶中可能会激活半胱氨酸蛋白酶样蛋白酶，从而建立细胞凋亡途径。由于暴露于50-100 μM FA剂量后在植物根细胞中形成的核出芽球似乎在胞质溶胶内被消化，我们建议将其标记为内部凋亡小体（IAB），这可能比以前使用的术语“凋亡样小体”提供更多信息。
Programmed cell death (PCD), now known as apoptosis, is accompanied by specific morphological features. In this study, fusaric acid, afusariummycotoxin, was used to examine cell death in saffron (Crocus sativusLinnaeus) roots, using several apoptosis assays. Our results show that moderate FA doses (50–100 μM) induce apoptotic features while high FA doses (> 200 μM) stimulate necrosis. The apoptotic-like features induced by moderate doses of FA include chromatin condensation, formation of condensed chromatin spheres which bud from the nucleus, fragmentation of nucleosomal DNA into ∼ 180 bp fragments, exposure of phosphatidyl serine to the external membrane leaflet, delivery of cytochromecto cytosol, and generation of H2O2. These apoptotic alterations in root cells are not observed in the presence of serine protease, caspase-1 or caspase-3 inhibitors. It is proposed that production of H2O2and release of cytochromecinto the cytosol may activate caspase-like proteases and thus establish the apoptotic pathway. As nuclei budding spheres formed in plant root cells after exposure to 50–100 μM FA doses seem to be digested inside the cytosol, we suggest labeling them as internal apoptotic bodies (IAB) that may be more informative than previously used term, apoptotic-like bodies.