Adaptive immunity to nontypeable Haemophilus influenzae
Adaptive immunity to nontypeable Haemophilus influenzae
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DOI：
10.1164/rccm.200207-728oc
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发表时间：
2003-02-15
影响因子：
24.7
通讯作者：
Holdsworth, SR
中科院分区：
文献类型：
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作者：
King, PT;Hutchinson, PE;Holdsworth, SR
无法分型的流感嗜血杆菌（NTHi）定植于大多数健康人的上呼吸道，也是慢性阻塞性肺病感染的主要原因。对这种细菌的免疫反应尚未得到很好的表征。我们检验了NTHi反复气道感染可能与非清除性适应性免疫相关的假设。研究对象为健康对照组和患有严重慢性H。流感。我们确定，两组中的所有受试者都有可检测到的NTHi抗体，这表明大多数正常人都产生了适应性免疫反应。为了表征免疫应答的性质，我们通过流式细胞术和外周血中的免疫球蛋白亚类水平测量了T辅助细胞细胞因子和CD40配体的抗原特异性产生。我们发现，正常对照组对NTHi的反应具有明显的CD40配体产生。相反，支气管扩张症患者主要产生Th2细胞因子，CD40配体表达减少，产生不同的免疫球蛋白G亚类。因此，慢性感染NTHi支气管扩张症与适应性免疫的变化，可能是重要的支气管感染的发病机制。
Nontypeable Haemophilus influenzae (NTHi) colonizes the upper respiratory tract of most healthy people and is also a major cause of infection in chronic obstructive lung disease. The immune response to this bacterium has not been well characterized. We tested the hypothesis that recurrent airway infection with NTHi may be associated with nonclearing adaptive immunity. Study subjects were healthy control subjects and patients with idiopathic bronchiectasis who had severe chronic infection with H. influenzae. We established that all subjects in both groups had detectable antibody to NTHi, suggesting that most normal people have developed an adaptive immune response. To characterize the nature of the immune response, we measured antigen-specific production of T helper cell cytokines and CD40 ligand by flow cytometry and immunoglobulin subclass levels in peripheral blood. We found that normal control subjects made The response to NTHi with distinct CD40 ligand production. In contrast, subjects with bronchiectasis had predominant production of Th2 cytokines, decreased expression of CD40 ligand, and different immunoglobulin G subclass production. Therefore, chronic infection with NTHi in bronchiectasis is associated with a change in adaptive immunity that may be important in the pathogenesis of bronchial infection.