Effects of suprachiasmatic transplants on circadian rhythms of neuroendocrine function in golden hamsters
Effects of suprachiasmatic transplants on circadian rhythms of neuroendocrine function in golden hamsters
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DOI：
10.1210/en.140.1.207
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发表时间：
1999-01-01
期刊：
影响因子：
4.8
通讯作者：
Bittman, EL
中科院分区：
文献类型：
--
作者：
Meyer-Bernstein, EL;Jetton, AE;Bittman, EL
移植胎儿组织，包括视交叉上核（SCN）的下丘脑恢复运动节律的行为mammic，SCN损伤的叙利亚仓鼠。我们试图确定这种移植是否也恢复SCN损伤仓鼠的内分泌节律。在实验1中，SCN损伤中断发情周期在14小时的光，10小时黑暗的光周期和运动节律在恒定的昏暗的红光（DD）。SCN移植，恢复行为昼夜节律始终未能重建发情周期。卵巢切除术后，雌二醇植入物引发LH激增在大约昼夜节律的时间8在10个12脑完整的控制女性和0的9 SCN损伤，移植的女性。日常节奏的主要尿褪黑激素代谢产物，6 α-硫酸褪黑激素，没有重新建立的行为功能移植。在实验2中，SCN损伤消除了维持在DD中的成年雄性仓鼠的运动节律性。7至12周后，恢复自发活动节律的胎儿移植，主机和假损伤的控制被斩首在昼夜节律时间4，8，12，16，20，或24。明确的昼夜节律的血清皮质酮和皮质醇被视为假损伤的男性，在晚主观一天的高峰。无昼夜节律的肾上腺激素在血清中明显病变移植男性。睾丸退化，观察到在完整的和假损伤的男性保持在DD，是不存在的，不仅在移植大脑皮层的mucosacn-lesioned仓鼠，而且在动物中，下丘脑移植恢复运动节律。松果体褪黑激素的浓度急剧上升，在主观的夜晚在控制仓鼠，但不是在SCN-损伤的动物轴承行为有效transplants.Even恢复的SCN移植的运动活动的昼夜节律，昼夜内分泌节律没有重新建立。内分泌节律可能需要与胎儿移植物所建立的SCN输出在性质上不同或更广泛。
Grafts of fetal tissue including the suprachiasmatic nucleus (SCN) of the hypothalamus restore locomotor rhythmicity to behaviorally arrhythmic, SCN-lesioned Syrian hamsters. We sought to determine whether such transplants also reinstate endocrine rhythms in SCN-lesioned hamsters. In Exp 1, SCN lesions interrupted estrous cycles in a 14 h light, 10 h dark photoperiod and locomotor rhythms in constant dim red light (DD). SCN grafts that reinstated behavioral circadian rhythms consistently failed to reestablish estrous cycles. After ovariectomy, estradiol implants triggered LH surges at approximately circadian time 8 in 10 of 12 brain-intact control females and 0 of 9 SCN-lesioned, grafted females. Daily rhythms of the principal urinary melatonin metabolite, 6 alpha-sulfatoxymelatonin, were not reestablished by behaviorally functional grafts. In Exp 2, SCN lesions eliminated locomotor rhythmicity in adult male hamsters maintained in DD. Seven to 12 weeks after restoration of locomotor activity rhythms by fetal grafts, hosts and sham-lesioned controls were decapitated at circadian times 4, 8, 12, 16, 20, or 24. Clear circadian rhythms of both serum corticosterone and cortisol were seen in sham-lesioned males, with peaks in late subjective day. No circadian rhythms in either adrenal hormone were evident in serum from lesioned-grafted males. Testicular regression, observed in intact and sham-lesioned males maintained in DD, was absent not only in arrhythmic SCN-lesioned hamsters given grafts of cerebral cortex, but also in animals in which hypothalamic grafts had reinstated locomotor rhythmicity. The pineal melatonin concentration rose sharply during the late subjective night in control hamsters, but not in SCN-lesioned animals bearing behaviorally effective transplants.Even though circadian rhythms of locomotor activity are restored by SCN transplants, circadian endocrine rhythms are not reestablished. Endocrine rhythms may require qualitatively different or more extensive SCN outputs than those established by fetal grafts.