Interaction of antibody with Forssman antigen in guinea pigs. A mechanism of adaptation to antibody- and complement-mediated injury.
Interaction of antibody with Forssman antigen in guinea pigs. A mechanism of adaptation to antibody- and complement-mediated injury.
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发表时间：
1995-05
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通讯作者：
Yukio Yuzawa;J. Brett;A. Fukatsu;S. Matsuo;P. R. Caldwell;N. Niesen;F. Milgrom;G. Godman;D. S
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作者：
Yukio Yuzawa;J. Brett;A. Fukatsu;S. Matsuo;P. R. Caldwell;N. Niesen;F. Milgrom;G. Godman;D. S
Forssman抗原是一种具有抗原特异性的鞘糖脂，由膜外半抗原糖决定，类似于血型抗原和异种器官移植的主要屏障抗原。在此，我们描述了Forssman抗原在豚鼠肺和肾中的定位及其与抗体在体外和体内（Forssman反应）相互作用的后果。培养的豚鼠主动脉内皮细胞暴露于Forssman抗体诱导抗原-抗体复合物在细胞表面的快速重新分布，随后通过质膜起泡作为囊泡或片段发生脱落，并与细胞表面抗原的消失相关（抗原调节）。豚鼠存活频繁静脉感染增加量的抗体，共20至40致死剂量，开发了一个部分或完全适应广义福斯曼反应，适应与部分或完全调制福斯曼抗原在肺表面，并在较小程度上，肾内皮和上皮细胞。这些发现支持了这样的假设，即内皮碳水化合物抗原的调节有助于适应高度血管化的器官暴露于耐受水平的同种或异种抗体。
Forssman antigen is a glycosphingolipid with antigenic specificity determined by extra-membrane haptenic sugars similar to blood group antigens and antigens that are the main barrier to xenogeneic organ transplantation. Herein, we describe the localization of Forssman antigen in guinea pig lungs and kidneys and the consequences of its interaction with antibodies in vitro and in vivo (Forssman reaction). Exposure of cultured guinea pig aortic endothelial cells to Forssman antibodies induced rapid redistribution of antigen-antibody complexes at the cell surface, followed by shedding that occurred by blebbing of plasma membrane as vesicles or fragments, and was associated with disappearance of antigen from the cell surface (antigenic modulation). Guinea pigs surviving frequent intravenous infections of increasing amounts of antibodies, for a total of 20 to 40 lethal doses, developed a partial or complete adaptation to generalized Forssman reaction, and adaptation was associated with partial or complete modulation of Forssman antigen at the surface of the pulmonary and, in minor degree, renal endothelial and epithelial cells. These findings support the hypothesis that modulation of endothelial carbohydrate antigens contributes to adaptation of highly vascularized organs exposed to tolerable levels of allo- or xenoantibodies.