Development of Artificial-Sebum-Containing Leeming and Notman Agar Medium to Enhance the Growth of Malassezia
Development of Artificial-Sebum-Containing Leeming and Notman Agar Medium to Enhance the Growth of Malassezia
复制标题
开发含人工皮脂的 Leeming 和 Notman 琼脂培养基以促进马拉色菌的生长
DOI：
10.1007/s11046-022-00634-9
复制
发表时间：
2022
期刊：
影响因子：
5.5
通讯作者：
Sugita T
中科院分区：
文献类型：
--
作者：
Suzuki K;Inoue M;Takayama K;Nagahama T;Cho O;Kurakado S;Sugita T
改良Leeming and Notman琼脂培养基(MLNA)已被广泛用于马拉色菌属嗜脂性真菌的培养。我们开发了一种新的含有皮脂的人工MLNA，以获得更高的马拉色菌产量。MLNA中的橄榄油被由甘油三酯(三油酸酯)、二甘油酯(硬脂酸甘油酯)、脂肪酸(棕榈酸、肉豆蔻酸、十五酸和油酸)和角鲨烯组成的人造皮脂取代。此外，吐温60还被自乳化硬脂酸甘油酯取代。9种与人类相关的马拉色菌在含皮脂的人工MLNA上生长良好，人体皮肤上最主要的真菌限制马拉色菌在人工含皮脂的MLNA中显示出两倍于标准MLNA中的湿细胞重量。
Modified Leeming and Notman agar medium (mLNA) has been widely utilized to grow lipophilic fungi belonging to the genusMalassezia. We developed a new artificial-sebum-containing mLNA to obtain higher yields ofMalasseziaspecies. The olive oil in mLNA was replaced with an artificial sebum composed of triglyceride (triolein), diglyceride (glyceryl distearate), fatty acids (palmitic acid, myristic acid, pentadecanoic acid, and oleic acid), and squalene. Furthermore, the Tween 60 was replaced with self-emulsifying glyceryl stearate. Nine human-associatedMalasseziaspecies grew well on the artificial-sebum-containing mLNA, and the most predominant fungus on human skin,Malassezia restricta, exhibited double wet cell weight in artificial sebum-containing mLNA compared to wet cell weight in standard mLNA.