Interferon induction by viruses. X. A model for interferon induction by Newcastle disease virus.
Interferon induction by viruses. X. A model for interferon induction by Newcastle disease virus.
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病毒诱导干扰素。
DOI：
10.1099/0022-1317-64-11-2419
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Sekellick,MJ
中科院分区：
文献类型：
--
作者：
Marcus,PI;Svitlik,C;Sekellick,MJ
如果细胞在体外“老化”6 至 7 天，未辐射的新城疫病毒（NDV，AV 株）会在初级鸡胚细胞中诱导高水平的干扰素 (IFN)。在抗NDV血清存在下进行的剂量（多重）反应（IFN产量）曲线以防止循环感染，结果表明NDV-AV库存中的IFN诱导颗粒（IFP）含量比传染性颗粒（PFP）多约七倍。这些非感染性 IFP 负责“衰老”细胞中几乎所有的 IFN 诱导，因为 PFP 被确定无法诱导 IFN。相比之下，以小鼠 L(Y) 细胞作为宿主，大约三分之一的 PFP 颗粒数量似乎被评分为 IFP。热和紫外线无论是在鸡细胞还是小鼠细胞中进行测试，辐射（254 nm）以相同的速率灭活 NDV IFP 和 PFP 活性，这意味着病毒体相关转录是诱导 IFN 所必需的。提出了一个模型来解释紫外线照射后传染性新城疫病毒产生干扰素诱导颗粒的情况。辐射，以及随后在高剂量辐射下失活。该模型定义了一系列 u.v. NDV 基因组中的靶点调节“未老化”鸡胚细胞中干扰素诱导颗粒活性的表达。
Unirradiated Newcastle disease virus (NDV, strain AV) induced high levels of interferon (IFN) in primary chick embryo cells if the cells were ‘aged’in vitrofor 6 to 7 days. Dose (multiplicity)-response (IFN yield) curves, carried out in the presence of anti-NDV serum to prevent cycling infection, revealed that stocks of NDV-AV contain about sevenfold more IFN-inducing particles (IFP) than infectious particles (PFP). These non-infectious IFP were responsible for nearly all IFN induction in ‘aged’ cells, since PFP were determined to be incapable of inducing IFN. In contrast, with mouse L(Y) cells as hosts, about one-third the number of particles as there are PFP appeared to score as IFP. Heat and u.v. radiation (254 nm) inactivated NDV IFP and PFP activity at the same rate whether tested in chick or mouse cells, implying that virion-associated transcription is required to induce IFN. A model is proposed to account for the generation of IFN-inducing particles from infectious NDV following u.v. irradiation, and their subsequent inactivation at high doses of radiation. The model defines a series of u.v. targets in the NDV genome that regulate the expression of IFN-inducing particle activity in ‘unaged’ chick embryo cells.