TOF-SIMS imaging of protein adsorption on dialysis membrane
TOF-SIMS imaging of protein adsorption on dialysis membrane
复制标题
DOI：
10.1016/j.apsusc.2004.03.149
复制
发表时间：
2004-06-15
影响因子：
6.7
通讯作者：
Kudo, M
中科院分区：
文献类型：
--
作者：
Aoyagi, S;Hayama, M;Kudo, M
飞行时间二次离子质谱仪(TOF-SIMS)能够对中空纤维透析膜等绝缘样品上的蛋白质进行化学成像。应减少蛋白在透析膜上的吸附造成的白蛋白损失和扩散通透性的降低，以提高患者的透析充分性。对吸附牛血清白蛋白(BSA)的中空纤维透析膜进行了研究。比较了天然膜和牛血清白蛋白吸附膜的TOF-SIMS图像和光谱，以确定与BSA和膜相关的二次离子。用信息论方法选取了与牛血清白蛋白及各膜相关的二次离子峰，并用主成分分析对其进行了表征。获得了BSA在天然膜和处理膜上吸附的化学图像，发现BSA的透过性和膜与BSA之间的相互作用肯定取决于膜的性质。用信息论获得的TOF-SIMS成像是估算蛋白质在透析膜上吸附的有力工具。(C)2004爱思唯尔B.V.保留所有权利。
Time-of-flight secondary ion mass spectrometry (TOF-SIMS) is capable of chemical imaging of proteins on insulated samples such as hollow-fiber dialysis membranes. Albumin loss and a lowering of diffusive permeability caused by protein adsorption on dialysis membranes should be reduced in order to enhance dialysis adequacy of the patients. Bovine serum albumin (BSA)adsorbed hollow-fiber dialysis membranes were tested in the present study. TOF-SIMS images and spectra of both native membranes and BSA-adsorbed membranes were compared in order to identify secondary ions related to BSA and membranes. Peaks of secondary ions related to BSA and each membrane were selected by means of information theory, and they are characterized by principal component analysis (PCA). Chemical images of BSA adsorption on both native and treated membranes were obtained to find that BSA permeability and interaction between the membranes and BSA definitely depend on the properties of a membrane. TOF-SIMS imaging obtained with information theory is a powerful tool to estimate protein adsorption on the dialysis membranes. (C) 2004 Elsevier B.V. All rights reserved.