Direct analysis of the extracellular proteome from two strains of Helicobacter pylori.
Direct analysis of the extracellular proteome from two strains of Helicobacter pylori.
复制标题
直接分析两种幽门螺杆菌菌株的细胞外蛋白质组。
DOI：
10.1002/pmic.200600875
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发表时间：
2007
期刊：
影响因子：
3.4
通讯作者：
Hoover,TimothyR
中科院分区：
文献类型：
--
作者：
Smith,ToddG;Lim,Jae-Min;Weinberg,MichaelV;Wells,Lance;Hoover,TimothyR
Helicobacter pyloriextracellular proteins are of interest because of possible roles in pathogenesis, host recognition, and vaccine development. We utilized a unique approach by growing two strains (including one nonsequenced strain) in a defined serum‐free medium and directly analyzing the proteins present in the culture supernatants by LC‐MS/MS. Over 125 proteins were identified in the extracellular proteomes of twoH. pyloristrains. Forty‐five of these proteins were enriched in the extracellular fraction when compared to soluble cell‐associated protein samples. Our analysis confirmed and expanded on the previously reportedH. pyloriextracellular proteome. Extracellular proteins of interest identified here includedcagpathogenicity island protein Cag24 (CagD); proteases HP0657 and HP1012; a polysaccharide deacetylase, HP0310, possibly involved in the hydrolysis of acetyl groups from hostN‐acetylglucosamine residues or from residues on the cell surface; and HP0953, an uncharacterized protein that appears to be restricted toHelicobacterspecies that colonize the gastric mucosa. In addition, our analysis found eight previously unidentified outer membrane proteins and two lipoproteins that could be important cell surface proteins.
