Multiple sex pheromones and receptors of a mushroom-producing fungus elicit mating in yeast
Multiple sex pheromones and receptors of a mushroom-producing fungus elicit mating in yeast
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DOI：
10.1091/mbc.10.8.2559
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发表时间：
1999-08-01
影响因子：
3.3
通讯作者：
Raper, CA
中科院分区：
文献类型：
--
作者：
Fowler, TJ;DeSimone, SM;Raper, CA
产蘑菇的裂褶菌有数千种交配类型，部分由大量脂肽信息素和它们的G蛋白连接受体定义。不同交配类型编码的信息素和受体的兼容组合调节性发育途径，导致蘑菇形成和减数分裂。一组复杂的信息素-受体相互作用最大限度地增加了异种繁殖的可能性；例如，一个信息素可以激活一个以上的受体，一个受体可以被一个以上的信息素激活。目前的研究表明，裂殖子菌性信息素及其受体在酿酒酵母中表达后，可以替代内源信息素及其受体，通过酵母G蛋白诱导酵母信息素应答途径。活性裂殖酵母信息素的分泌需要酵母脂肽信息素a因子所使用的生物合成机制的一部分，但不是全部。裂殖子中信息素-受体对之间相互作用的特异性在酵母中被复制，从而为探索一类在广泛的生物体中常见的七个跨膜区受体的信息素-受体相互作用的分子方面提供了一个强大的系统。
The mushroom-producing fungus Schizophyllum commune has thousands of mating types defined, in part, by numerous lipopeptide pheromones and their G protein-linked receptors. Compatible combinations of pheromones and receptors encoded by different mating types regulate a pathway of sexual development leading to mushroom formation and meiosis. A complex set of pheromone-receptor interactions maximizes the likelihood of outbreeding; for example, a single pheromone can activate more than one receptor and a single receptor can be activated by more than one pheromone. The current study demonstrates that the sex pheromones and receptors of Schizophyllum, when expressed in Saccharomyces cerevisiae, can substitute for endogenous pheromone and receptor and induce the yeast pheromone response pathway through the yeast G protein. Secretion of active Schizophyllum pheromone requires some, but not all, of the biosynthetic machinery used by the yeast lipopeptide pheromone a-factor. The specificity of interaction among pheromone-receptor pairs in Schizophyllum was reproduced in yeast, thus providing a powerful system for exploring molecular aspects of pheromone-receptor interactions for a class of seven-transmembrane-domain receptors common to a wide range of organisms.