Structure elucidation of a senescence cross-link from human extracellular matrix. Implication of pentoses in the aging process.
Structure elucidation of a senescence cross-link from human extracellular matrix. Implication of pentoses in the aging process.
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发表时间：
1989-12
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通讯作者：
D. Sell;V. Monnier
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作者：
D. Sell;V. Monnier
从人细胞外基质中分离和结构解析耐酸荧光分子揭示了咪唑并[4，5-B]吡啶鎓分子的存在，所述咪唑并[4，5-b]吡啶鎓分子包含由戊糖交联的赖氨酸和精氨酸残基。通过核糖与赖氨酸和精氨酸残基的非酶反应在体外实现结构确认。这种名为pentosidine的交联物也可以用核糖、阿拉伯糖、木糖和来苏糖的异构体合成，也可以在37摄氏度下将年轻的人类胶原蛋白与这些糖一起孵育。在多种人体组织中发现了戊糖苷，包括血浆蛋白和红细胞。它在培养细胞中的存在强烈表明核糖或核糖核苷酸代谢物是前体。戊糖介导的蛋白质交联的意外发现提出了关于衰老过程的新问题。
Isolation and structure elucidation of an acid-resistant fluorescent molecule from human extracellular matrix revealed the presence of an imidazo[4,5-b]pyridinium molecule comprising a lysine and an arginine residue cross-linked by a pentose. Structure confirmation was achieved in vitro by the nonenzymatic reaction of ribose with lysine and arginine residues. The cross-link, named pentosidine, could also be synthesized with isomers of ribose, arabinose, xylose, and lyxose as well as by incubating young human collagen with these sugars at 37 degrees C. Pentosidine was found in a variety of human tissues including plasma proteins and red blood cells. Its presence in cells grown in culture strongly suggests ribose or ribonucleotide metabolites as precursors. The unexpected discovery of pentose-mediated protein cross-linking raises new questions concerning the aging process.