Assessment of pig saliva as a Streptococcus suis reservoir and potential source of infection on farms by use of a novel quantitative polymerase chain reaction assay
Assessment of pig saliva as a Streptococcus suis reservoir and potential source of infection on farms by use of a novel quantitative polymerase chain reaction assay
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DOI：
10.2460/ajvr.79.9.941
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发表时间：
2018-09-01
影响因子：
1
通讯作者：
Sekizaki, Tsutomu
中科院分区：
文献类型：
--
作者：
Arai, Sakura;Kim, Hyunjung;Sekizaki, Tsutomu
目的了解猪链球菌和副猪链球菌在日本养猪场的定植情况，确定感染源。样本：来自4个农场84头不同生长阶段的健康猪的唾液、粪便和阴道拭子样本，以及这些农场饲料槽和饮水机的拭子样本。样本采集时间为2015年8月至2016年6月。设计了两种定量PCR （qPCR）检测方法（一种用于猪链球菌，另一种用于副猪链球菌）用于研究。新的qPCR检测方法与先前描述的猪链球菌血清2型或1/2型和总细菌的qPCR检测方法结合使用。评估每个样品中细菌的相对丰度。结果在所有唾液样本和部分其他样本中均检测到猪链球菌，而副猪链球菌在包括唾液样本在内的所有样本中均未检测到，说明定植偏好存在差异。猪链球菌占唾液总细菌的比例随着猪的年龄而增加。在2个猪链球菌感染流行的养殖场，在少量唾液样本和饲料槽拭子样本中检测到猪链球菌血清2型或1/2型。结论和临床意义：猪的唾液，尤其是母猪的唾液，是猪链球菌感染的宿主和传染源。本文所描述的qPCR检测方法可能提供一种监测猪链球菌的有效方法，从而为猪场的有效疾病控制提供依据。
OBJECTIVETo evaluate colonization of Streptococcus suis and Streptococcus parasuis on pig farms in Japan and to identify sources of infections.SAMPLESaliva, feces, and vaginal swab samples from 84 healthy pigs of several growth stages on 4 farms and swab samples of feed troughs and water dispensers at those farms.PROCEDURESSamples were collected from August 2015 to June 2016. Two quantitative PCR (qPCR) assays (one for S suis and the other for S parasuis) were designed for use in the study. The novel qPCR assays were used in combination with previously described qPCR assays for S suis serotype 2 or 1/2 and total bacteria. Relative abundance of bacteria in each sample was evaluated.RESULTSStreptococcus suis was detected in all saliva samples and some of the other samples, whereas S parasuis was not detected in any of the samples, including saliva samples, which indicated a difference in colonization preference. The ratio of S suis to total bacteria in saliva appeared to increase with age of pigs. Streptococcus suis serotype 2 or 1/2 was detected in a few saliva samples and feed trough swab samples at 2 farms where S suis infections were prevalent.CONCLUSIONS AND CLINICAL RELEVANCESaliva, especially that of sows, appeared to be a reservoir and source of S suis infection for pigs. The qPCR assay described here may provide an effective way to monitor for S suis in live pigs, which could lead to effective disease control on pig farms.