htert expression correlates with MYC over‐expression in human prostate cancer
htert expression correlates with MYC over‐expression in human prostate cancer
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DOI：
10.1002/(sici)1097-0215(20000320)89:2
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发表时间：
2000-03
影响因子：
6.4
通讯作者：
A. Latil;D. Vidaud;A. Valéri;G. Fournier;M. Vidaud;R. Lidereau;O. Cussenot;I. Biàche
中科院分区：
文献类型：
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作者：
A. Latil;D. Vidaud;A. Valéri;G. Fournier;M. Vidaud;R. Lidereau;O. Cussenot;I. Biàche
端粒酶催化亚单位（htert）的表达构成了人类癌症发展的关键步骤。虽然htert的调控机制尚不清楚，但一些研究表明c-myc可能激活其表达。前列腺癌是西方国家男性最常见的恶性肿瘤之一。由于myc的表达失调以及端粒酶活化可能有助于这种癌症的致病性，我们研究了前列腺肿瘤发生中的这一途径。为此，采用基于TaqMan方法学的真实的实时定量PCR测定，对33例散发性前列腺肿瘤中myc-和htert-mRNA表达进行定量。在33个肿瘤中的19个（58%）中观察到myc过度表达，而在22个（67%）中观察到通过htert表达评估的端粒酶状态。myc过度表达或htert表达水平与肿瘤分期或Gleason分级之间无相关性。myc过表达和htert表达升高之间存在显著相关性（p = 0.0024），表明在前列腺肿瘤中经常观察到的端粒酶活性上调可能是通过myc反式激活htert而实现的。c-myc蛋白刺激htert表达从而增强端粒酶活性的能力可能是前列腺肿瘤发生的重要步骤。国际癌症杂志（Pred. Oncol.）89：172-176，2000.© 2000 Wiley利斯公司
Expression of the telomerase catalytic sub‐unit (htert) constitutes a key step in the development of human cancer. Although htert regulation is still unclear, several studies suggest that c‐myc may activate its expression. Prostate cancer is one of the most common malignancies among men in Western countries. Since de‐regulated expression of myc as well as telomerase activation may contribute to the pathogenicity of this cancer, we investigated this pathway in prostate tumorigenesis. For this purpose, myc‐ and htert‐mRNA expression was quantified in 33 sporadic prostate tumors using a real‐time quantitative PCR assay based on TaqMan methodology. myc over‐expression was observed in 19 (58%) of 33 tumors, whereas telomerase status evaluated by htert expression was observed in 22 (67%). There was no correlation between myc over‐expression or htert expression level and tumor stage or Gleason grade. A significant association (p = 0.0024) was found between myc over‐expression and elevated htert expression, indicating that the up‐regulation of telomerase activity often observed in prostate tumors might be conferred through transactivation of htert by myc. It is likely that the ability of c‐myc protein to stimulate expression of htert and thereby enhance telomerase activity represents an important step in prostate tumorigenesis. Int. J. Cancer (Pred. Oncol.) 89:172–176, 2000. © 2000 Wiley‐Liss, Inc.