Evaluation of Rice stripe virus transmission efficiency by quantification of viral load in the saliva of insect vector
Evaluation of Rice stripe virus transmission efficiency by quantification of viral load in the saliva of insect vector
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通过昆虫媒介唾液中病毒载量的定量评估水稻条纹病毒传播效率
DOI：
10.1002/ps.5311
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发表时间：
2019-07-01
影响因子：
4.1
通讯作者：
Cui, Feng
中科院分区：
文献类型：
--
作者：
Li, Jing;Zhao, Wan;Cui, Feng
背景在昆虫进食期间，持久性植物病毒从昆虫肠道转移到血淋巴，最终转移到唾液腺，然后用唾液接种到植物宿主中。病毒在唾液中的积累是昆虫媒介传播能力的重要指标。为了评价灰飞虱对水稻条纹病毒（rice stripe virus，RSV）的传播能力，采用绝对实时定量PCR方法，通过定量测定病毒基因拷贝数，成功地测定了灰飞虱唾液中RSV的积累量。结果在人工饲料中取食24 h后，仅10只带毒和10只非带毒飞虱感染RSV 7 d后的唾液中就可检测到病毒衣壳蛋白（CP）和疾病特异性蛋白（SP）基因的拷贝数。当稻飞虱G蛋白途径抑制因子2或c-Jun N-末端激酶的表达被敲低时，唾液中CP和SP的拷贝数也随之发生变化。结论本研究为评价褐飞虱对RSV的传播效率提供了一种准确、简便的检测方法，该方法也适用于其他持久性植物病毒的检测。(c)2019化学工业协会
BACKGROUND Persistent plant viruses transfer from insect gut to the hemolymph, and finally to the salivary glands before inoculation into the plant hosts with saliva during insect feeding. Virus accumulation in saliva is an important indicator for the transmission ability of an insect vector. In order to evaluate the transmission ability of the small brown planthopper to rice stripe virus (RSV), we successfully measured accumulation of RSV in the saliva of planthoppers via the absolute real-time quantitative polymerase chain reaction method by quantifying the copy numbers of viral genes. RESULTS After feeding on an artificial diet for 24 h, the copy numbers of viral genes of capsid protein (CP) and disease-specific protein (SP) can be detected in the saliva collected from as few as ten viruliferous planthoppers and ten non-viruliferous planthoppers after infected with RSV for 7 days. When the expression of planthopper G protein pathway suppressor 2 or c-Jun N-terminal kinase was knocked down, the copy numbers of CP and SP in the saliva varied accordingly. CONCLUSION Our study provided an accurate and convenient detection system to evaluate the transmission efficiency of RSV by small brown planthoppers, and this method may also be suitable for other persistent plant viruses. (c) 2019 Society of Chemical Industry