Coordinated expression of ncRNAs and HOX mRNAs in the human HOXA locus
Coordinated expression of ncRNAs and HOX mRNAs in the human HOXA locus
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DOI：
10.1016/j.bbrc.2007.03.200
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发表时间：
2007-06-08
影响因子：
3.1
通讯作者：
Hirose, Tetsuro
中科院分区：
文献类型：
--
作者：
Sasaki, Yasnory T. F.;Sano, Miho;Hirose, Tetsuro
在人HOXA基因座中，许多ncRNA以与HOXA mRNA相反的方向从基因间区域转录。我们观察到ncRNA和HOXA蛋白的基因组结构在人和小鼠之间是高度保守的。我们研究了这些ncRNA和HOXA mRNA在各种人体组织中的表达谱。人组织中ncRNA的表达模式与沿前后轴共沿着线性表达的相邻HOXA mRNA的表达模式一致。甚至在转化的肿瘤和癌细胞系中也观察到这种协调表达，表明ncRNA的表达是HOXA基因表达调控的先决条件。从HOXA簇的最上游位置转录的HIT 18844 ncRNA具有潜在地形成进化上保守的二级结构的超保守的短片段。我们的数据表明ncRNA在HOXA基因表达的调控中起关键作用。(c)2007年爱思唯尔公司All rights reserved.
In the human HOXA locus a number of ncRNAs are transcribed from the intergenic regions in the opposite direction to HOXA mRNAs. We observed that the genomic organization of genes for the ncRNAs and HOXA proteins is highly conserved between human and mouse. We examined the expression profiles of these ncRNAs and HOXA mRNAs in various human tissues. The expression patterns of ncRNAs in human tissues coincide with those of the adjacent HOXA mRNAs that are collinearly expressed along the anteroposterior axis. This coordinated expression was observed even in transformed tumors and cancer cell lines, suggesting that the expression of ncRNAs is prerequisite for the regulated expression of HOXA genes. HIT 18844 ncRNA transcribed from the most upstream position of the HOXA cluster possesses an ultra-conserved short stretch which potentially forms an evolutionarily conserved secondary structure. Our data suggest a critical role for ncRNAs in the regulation of HOXA gene expression. (c) 2007 Elsevier Inc. All rights reserved.