Genetic diversity and systematic relationships in sweetpotato (Ipomoea batatas (L.) Lam.) and related species as revealed by RAPD analysis
Genetic diversity and systematic relationships in sweetpotato (Ipomoea batatas (L.) Lam.) and related species as revealed by RAPD analysis
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DOI：
10.1007/bf00051633
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发表时间：
1994-01-01
影响因子：
2
通讯作者：
Austin, D. F.
中科院分区：
文献类型：
--
作者：
Jarret, R. L.;Austin, D. F.
使用 15 个 10mer 引物与 Stoffel 片段结合，检测来自大洋洲、秘鲁、菲律宾和美国的 26 个甘薯 (I. batatas (L.) Lam.) 种质以及来自 Batatas 区的 8 个甘薯种之间的随机扩增多态性 DNA (RAPD)。对六倍体 I. batatas 中检测到的 56 个多态性进行表型和主坐标分析，清楚地描绘了南太平洋和秘鲁甘薯品系。这两个美国品种与海洋材料聚集在一起。对 8 个番薯属物种的分支和表型分析支持之前发表的基于形态学和 RFLP 数据的系统发育。在检查的物种中，I. tabascana、I. trifida 和来自墨西哥和厄瓜多尔的 I. batatas 的四倍体形式，包括 L. batatas var. apiculata 是与栽培六倍体 I. batata 最密切相关的分类单元。这些发现支持 RAPD 标记在评估甘薯遗传多样性和建立甘薯分类学和进化关系方面的实用性。
Fifteen 10mer primers, in combination with the Stoffel fragment, were used to detect random amplified polymorphic DNA (RAPDs) among 26 accessions of sweetpotato (I. batatas (L.) Lam.) from Oceania, Peru, the Philippines, and the United States and between 8 Ipomoea species from section Batatas. Phenetic and principal coordinate analysis of the 56 polymorphisms detected within the hexaploid I. batatas clearly delineated the South Pacific and the Peruvian sweetpotato lines. The two U.S. cultivars clustered with the Oceanic materials. Cladistic and phenetic analysis of 8 Ipomoea species supports previously published phylogenies based on morphological and RFLP data. Among the species examined, I. tabascana, I. trifida and the tetraploid forms of I. batatas from Mexico and Ecuador, including L. batatas var. apiculata, are the taxa most closely related to the cultivated hexaploid I. batatas. These findings support the utility of RAPD markers for evaluating genetic diversity in sweetpotato and for establishing taxonomic and evolutionary relationships in Ipomoea.