Study of Biochemical Pathway and Enzyme Involved in Metsulfuron-Methyl Degradation by Ancylobacter sp XJ-412-1 Isolated from Soil
Study of Biochemical Pathway and Enzyme Involved in Metsulfuron-Methyl Degradation by Ancylobacter sp XJ-412-1 Isolated from Soil
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土壤中Ancylobacter sp XJ-412-1降解甲磺隆的生化途径及酶的研究
DOI：
10.1007/s00284-011-9919-z
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发表时间：
2011-06-01
影响因子：
2.6
通讯作者：
Huang, Xing
中科院分区：
文献类型：
--
作者：
Lu, Peng;Jin, Lei;Huang, Xing
Ancylobacter sp. XJ-412-1, capable of degrading metsulfuron-methyl, was isolated from sulfonylurea-contaminated soil. When metsulfuron-methyl was provided as the sole carbon source, more than 90.5% of metsulfuron-methyl at concentration of 50 mg l(-1) was degraded by strain XJ-412-1 after incubation at 30 degrees C for 7 days. The initial degradation products of metsulfuron-methyl (MSM), thifensulfuron-methyl (TSM), and bensulfuron-methyl (BSM) by XJ-412-1 were identified as corresponding deesterified derivatives by liquid chromatography-mass spectrometry, which indicated a primary pathway of the deesterification of these three sulfonylurea herbicides. The carboxyesterase activity of the cell-free extracts was assayed and strongly inhibited by 4-chloromercuribenzoic acid (PCMB), diethyl pyrocarbonate (DEPC), phenylmethylsulfonyl fluoride (PMSF), and malathion.
