Influence of stereotaxically injected scrapie on neurotransmitter systems of mouse cerebellum.
Influence of stereotaxically injected scrapie on neurotransmitter systems of mouse cerebellum.
复制标题
立体定向注射痒病对小鼠小脑神经递质系统的影响。
DOI：
10.1016/0006-8993(88)91192-4
复制
发表时间：
1988
期刊：
影响因子：
2.9
通讯作者：
Carp,RI
中科院分区：
文献类型：
--
作者：
Quinn,MR;Kim,YS;Lossinsky,AS;Carp,RI
将 22L 痒病株立体定位注射到 C57BL/6J 小鼠的小脑中，以确定其在疾病早期临床阶段对该病的多个小脑神经递质系统的影响。在该模型中，空泡病变仅限于小脑，其他脑区域没有空泡化的证据。尽管病变遍及小脑的所有细胞层，但颗粒细胞层最为严重。在受瘙痒病影响的小鼠中观察到小脑重量、谷氨酸脱羧酶活性和N6-[腺嘌呤-2,8-3H]环己基腺苷结合位点的亲和力适度但显着（P<0.01）降低。高亲和力和低亲和力腺苷受体的密度不受影响。小脑中的腺苷受体高度定位于谷氨酸能、GABA 受体颗粒细胞的轴突末端。 GABA、苯二氮卓类、谷氨酸和毒蕈碱胆碱能受体显着改变。此外，谷氨酸的高亲和力摄取和胆碱乙酰转移酶的活性没有显着改变。 GABA 高亲和力摄取略有增加。尽管小脑的颗粒细胞层经历了严重的空泡化，但神经递质的变化却很明显。尽管这些结果表明痒病病理学与神经递质系统的完整性之间存在微妙的关系，但未受损的神经元群体中的代偿性神经化学变化可能掩盖了潜在的特定神经递质效应。
The 22L strain of scrapie was injected stereotaxically into the cerebellum of C57BL/6J mice to determine its effect on several cerebellar neurotransmitter systems during the early clinical stages of the disease. In this model vacuolar lesions are restricted to the cerebellum with no evidence of vacuolization in other brain regions. Although lesions develop throughtout all cell layers of the cerebellum, they are most severe in the granule cell layer. Modest but significant (P < 0.01) reductions in cerebellar weight, glutamate decarboxylase activity, and in the affinity of theN6-[adenine-2,8-3H]cyclohexyladenosine binding sites, were observed in scrapie affected mice. The densities of the high- and low-affinity adenosine receptors were unaffected. Adenosine receptors in the cerebellum are highly localized to the axon terminals of the glutamatergic, GABA receptive granule cells. GABA, benzodiazepine, glutamate, and muscarinic cholinergic receptors were significantly altered. In addition, the high-affinity uptake of glutamate, and the activity of choline acetyltransferase were not significantly changed. GABA high-affinity uptake was slightly increased. Even though the granule cell layer of the cerebellum had undergone severe vacuolation, only modest neurotransmitter changes were apparent. Although these results suggest a tenous relationship between scrapie pathology and the integrity of neurotransmitter systems, it is possible that compensatory neurochemical changes in uncompromised neuronal populations may have masked potentially specific neurotransmitter effects.