MK-801 inhibits the micturition reflex in chronic bladder irritation caused by crystalluria in the
MK-801 inhibits the micturition reflex in chronic bladder irritation caused by crystalluria in the
复制标题
DOI：
10.1016/s1566-0702(02)00289-8
复制
发表时间：
2003-04-30
影响因子：
2.7
通讯作者：
Kawatani, M
中科院分区：
文献类型：
--
作者：
Ishida, F;Sato, T;Kawatani, M
通过尿动力学和药理学研究，探讨了晶体对排尿反射的影响以及对排尿能传递的影响。口服给予LP-805（100 mg/kg/天）12天的大鼠排泄出晶体。这些晶体（LP-805尿液）的pH值与正常尿液相同。晶体量为70-100个/格放大400倍。水晶的末端很锋利。膀胱内注射LP-805可引起正常大鼠排尿反射亢进。当输注溶液从盐水变为LP-805尿液时，单次膀胱测压（CMG）中的潜伏期降低至对照的57.6 +/- 2.1%，或连续CMG中降低至对照的51.4 +/- 0.9%。在单次CMG中，排尿量减少至对照的52.1 +/- 3.6%，或在连续CMG中减少至对照的615 +/- 0.8%。除去LP-805尿液后，这些参数恢复。在LP-805处理的大鼠中，膀胱内给予乙酸未诱导排尿反射亢进。这些数据表明，针刺晶体的慢性刺激可引起排尿反射亢进，从而增加传入神经元的活动，静脉注射MK-801（0.001 ~ 1 mg/kg）呈剂量依赖性地抑制排尿反射。LP-805处理大鼠（0.03 mg/kg i. v.）低于正常大鼠（0.56 mg/kg i. v.）。慢性刺激膀胱上皮后，MK-801对排尿反射的敏感性增强。这些数据表明，晶体引起的反射亢进的排尿反射，介导的NMDA受体。(C)2002 Elsevier Science B. V.保留所有权利。
Urodynamic and pharmacological studies were performed to investigate the effect of crystalluria on the micturition reflex and the involvement of glutamatergic transmission. The rats, which were given LP-805 (100 mg/kg/day) orally for 12 days, voided crystalluria. The pH of these crystalluria (LP-805 urine) was the same as normal urine. The amount of crystals was 70-100/division magnified 400 X. The end of the crystals was sharp. Intravesical administration of LP-805 urine induced hyperreflexia of the micturition reflex in normal rats. When the infusion solution was changed to LP-805 urine from saline, the latency was reduced to 57.6 +/- 2.1% of control in single cystometrogram (CMG) or was reduced to 51.4 +/- 0.9% of control in continuous CMG. The voiding volume was reduced to 52.1 +/- 3.6% of control in single CMG or was reduced to 615 +/- 0.8% of control in continuous CMG. These parameters were recovered after LP-805 urine was removed. Intravesical administration of acetic acid did not induce hyperreflexia of the micturition reflex in LP-805-treated rats. These data suggest that the chronic irritation by aculeate crystals might induce hyperreflexia of the micturition reflex, which increase afferent neuronal activity.Intravenous administration of MK-801 (0.001 to I mg/kg) inhibited the micturition reflex in a dose-dependent manner. The ID50 in LP-805-treated rats (0.03 mg/kg i.v.) was lower than that in normal rats (0.56 mg/kg i.v.). After chronic irritation of the bladder epithelium, MK-801 sensitivity was enhanced for the micturition reflex. These data suggested that crystalluria elicit hyperreflexia in the micturition reflex that mediated with NMDA glutamatergic receptors. (C) 2002 Elsevier Science B.V. All rights reserved.