The human herpesvirus-8 (Kaposils sarcoma-associated herpesvirus) ORF 40/41 region encodes two distinct transcripts
The human herpesvirus-8 (Kaposils sarcoma-associated herpesvirus) ORF 40/41 region encodes two distinct transcripts
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DOI：
10.1099/0022-1317-83-1-189
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发表时间：
2002-01-01
影响因子：
3.8
通讯作者：
Pari, GS
中科院分区：
文献类型：
--
作者：
AuCoin, DP;Pari, GS
编码ORF 40/41的人类疱疹病毒8（HHV-8）基因座是编码推定的primase相关因子的Epstein-Barr病毒BBLF 2/3基因的候选同源物。北方杂交结果显示，两个转录本来自HHV-8 ORF 40/41区域。这些转录本的大小（2.2和0.7 kb）表明，一个转录本是ORF 40和41剪接形式的结果，第二个转录本来自ORF 41内的区域。cDNA序列分析和5' RACE分析表明，ORF 40和41之间的内含子和ORF 40上游82 nt处的转录起始位点被去除。较小转录物的转录起始点被定位在ORF 41内。将转录起始位点上游的区域亚克隆到荧光素酶报告载体中，并且瞬时荧光素酶测定表明不同的启动子驱动每个转录物的表达。
The human herpesvirus-8 (HHV-8) locus encoding ORFs 40/41 is a candidate homologue for the Epstein-Barr virus BBLF 2/3 gene, which encodes the putative primase-associated factor. Northern blot data revealed that two transcripts originated from the HHV-8 ORF 40/41 region. The sizes of these transcripts (2.2 and 0.7 kb) suggested that one transcript was the result of a spliced form of ORFs 40 and 41 and the second transcript originated from a region within ORF 41. cDNA sequence and 5' RACE analysis revealed the removal of an intron between ORFs 40 and 41 and a transcriptional start site 82 nt upstream of ORF 40. The start of transcription for the smaller transcript was mapped to within ORF 41. Regions upstream of the transcriptional start sites were subcloned into a luciferase reporter vector, and transient luciferase assays indicated that distinct promoters drive the expression of each transcript.