Gizzard formation and the role of Bapx1
Gizzard formation and the role of Bapx1
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DOI：
10.1006/dbio.2000.0151
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发表时间：
2001-03-01
影响因子：
2.7
通讯作者：
Roberts, DJ
中科院分区：
文献类型：
--
作者：
Nielsen, C;Murtaugh, LC;Roberts, DJ
前-后肠模式由三个广泛的区域形成：前肠、中肠和后肠，它们具有不同的功能、形态和分子边界。胃划定了前肠的后边界。鸟类的胃由两个腔室组成：前腔室（前胃）和厚肌的后腔室（砂囊）。候选模式形成控制因子的表达被限制在鸡胃区域中，使得Bmp 4和Wnt 5a在砂囊中不表达。我们先前暗示Bmp 4控制肠道肌肉组织的生长和分化。BMP 4在发育中的砂囊中不表达，但在肠道的其余部分（包括相邻的前胃和中肠）中表达。Bapx 1：（Nkx3.2）在肌胃中表达，但在前胃或中肠中不表达。我们发现Bapx 1在腺胃中的异位表达导致了砂囊样形态，并抑制了Bmp 4和Wnt 5a的正常腺胃表达。反向功能Bapx 1构建体的过表达可导致小胃和Bmp 4和Wnt 5a表达到砂囊的异位延伸。我们认为Bapx 1的作用是调节Bmp 4和Wnt 5a的表达，从而形成鸟类胃的模式。(C)北京：科学出版社.
The anterior-posterior gut pattern is formed from three broad domains: fore-, mid-, and hindgut that have distinct functional, morphological, and molecular boundaries. The stomach demarcates the posterior boundary of the foregut. Avian stomachs are composed of two chambers: the anterior chamber (proventriculus) and the thick muscular posterior chamber (gizzard). Expression of candidate pattern formation control factors are restricted in the chick stomach regions such that Bmp4 and Wnt5a are not expressed in the gizzard. We previously implicated Bmp4 as controlling growth and differentiation of the gut musculature. Bmp4 is not expressed in the developing gizzard but is expressed in the rest of the gut including the adjacent proventriculus and midgut. Bapx1: (Nkx3.2) is expressed in the gizzard musculature but not in the proventriculus or midgut. We show ectopic expression of Bapx1 in the proventriculus results in a gizzard-like morphology and inhibits the normal proventricular expression of Bmp4 and Wnt5a. Overexpression of a reverse-function Bapx1 construct can result in a small stomach and ectopic extension of Bmp4 and Wnt5a expression into the gizzard. We suggest the role of Bapx1 is to regulate the expression of Bmp4 and Wnt5a to pattern the avian stomach. (C) 2001 Academic Press.