Screening of Botanical Extracts for Repellence against Western Corn Rootworm Larvae
Screening of Botanical Extracts for Repellence against Western Corn Rootworm Larvae
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DOI：
10.1007/s10905-016-9571-3
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发表时间：
2016-07-01
影响因子：
1
通讯作者：
Vidal, S.
中科院分区：
文献类型：
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作者：
Brandl, M. A.;Schumann, M.;Vidal, S.
西方玉米根虫 (WCR)（Diabrotica virgifera virgifera LeConte）对各种管理措施产生抗性的能力需要开发新的管理方案。驱虫提取物可以作为 WCR 控制中的功效增强剂。本研究在无选择生物测定中调查了大蒜 (Allium sativum L.)、胡椒 (Capsicum sp. L.)、芦荟 (Aloe ferox Mill.)、印楝 (Azadirachta indica A. Juss) 和姜黄 (Curcuma longa L.) 提取物对 WCR 幼虫的潜在驱避作用。在初步筛选中，大蒜和姜黄被评估为最有前途的提取物，因为 WCR 幼虫避开大蒜/姜黄处理过的土壤。在rhizotron中进一步评估姜黄，以量化幼虫随时间的分布和行为变化。 WCR 幼虫在根系施用后避开姜黄处理过的土壤长达 0.05 m，导致扩散增加并在根管中形成多个簇。随后，幼虫在土壤中活跃活动的比例增加，而幼虫取食的比例显着减少。土壤中的空间和行为变化得出这样的结论：姜黄是 WCR 幼虫的驱避提取物。通过与杀虫物质或微生物拮抗剂的协同作用，姜黄的驱避作用可能进一步用于 WCR 管理。
The ability of western corn rootworm (WCR) (Diabrotica virgifera virgifera LeConte) to develop resistance to various management practices requires the development of new management options. Repellent extracts can act as efficacy enhancing agents in WCR control. The present study investigated the potential repellence of garlic (Allium sativum L.), pepper (Capsicum sp. L), cape aloe (Aloe ferox Mill.), neem (Azadirachta indica A. Juss) and turmeric (Curcuma longa L.) derived extracts against WCR larvae in a no-choice bioassays. In initial screenings garlic and turmeric were evaluated as the most promising extracts as WCR larvae avoided garlic / turmeric treated soil. Turmeric was further evaluated in a rhizotron set up to quantify larval distribution and behavioral changes over time. WCR larvae avoided turmeric treated soil up to 0.05 m from its application in the root system, resulting in an increased dispersal and the formation of multiple clusters in the rhizotron. The proportion of larvae actively moving in the soil subsequently increased, whereas larval feeding significantly decreased. The spatial and behavioral alterations in the soil lead to the conclusion that turmeric is a repellent extract for WCR larvae. Turmeric repellence might further be exploited for WCR management through a synergy with insecticidal substances or microbial antagonists.