High levels of viremia in hu-PBL-NOD-scid mice with HIV-1 infection.
High levels of viremia in hu-PBL-NOD-scid mice with HIV-1 infection.
复制标题
感染 HIV-1 的 hu-PBL-NOD-scid 小鼠出现高水平的病毒血症。
作者：
Y. Koyanagi;Y. Tanaka;R. Tanaka;N. Misawa;Y. Kawano;T. Tanaka;M. Miyasaka;M. Ito;Y. Ueyama;N. Yamamoto
我们研究了人淋巴细胞移植的相容性和对HIV-1感染的易感性在2个新的免疫缺陷小鼠。NOD/Shi-scid小鼠通过scid突变与NOD小鼠回交产生，而C57 BL/6-RAG 2（0/0）通过敲除RAG-2基因产生。在新的免疫缺陷小鼠品系中重建人T淋巴细胞。我们发现新的免疫缺陷小鼠品系比传统的C.B-17-scid小鼠更有效地接受人PBL移植和HIV-1感染。特别是在hu-PBL-NOD/Shi-scid菌株中，我们再现了与HIV-1初次感染后相当或显著高于HIV-1初次感染后的高水平HIV-1病毒血症。这些结果表明，我们的hu-PBL-NOD-scid动物是有用的研究激活机制的HIV-1复制在体内和初次感染后。
We studied the compatibility of human lymphocyte engraftment and susceptibility to HIV-1 infection in 2 new immunodeficient mice. NOD/Shi-scid mice were generated by backcrossing of the scid mutation into NOD mice while C57BL/6-RAG2(0/0) were generated by knocking out the RAG-2 gene. Human T lymphocytes were reconstituted in new immunodeficient mouse strains. We found that the new immunodeficient mouse strains accepted human PBL engraftment and HIV-1 infection more efficiently than conventional C.B-17-scid mice. Especially in the hu-PBL-NOD/Shi-scid strain, we reproduced the high levels of HIV-1 viremia comparable to or at significantly higher levels than after HIV-1 primary infection. These results indicate that our hu-PBL-NOD-scid animal is useful for investigations of the activation mechanism in HIV-1 replication in vivo and after primary infection.