Effects of fasting and re-feeding on the expression of Dec1, Per1, and other clock-related genes
Effects of fasting and re-feeding on the expression of Dec1, Per1, and other clock-related genes
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DOI：
10.1093/jb/mvj165
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发表时间：
2006-09-01
影响因子：
2.7
通讯作者：
Kato, Yukio
中科院分区：
文献类型：
--
作者：
Kawamoto, Takeshi;Noshiro, Mitsuhide;Kato, Yukio
为了阐明外周生物钟系统的食物依赖性振荡机制，我们研究了禁食对小鼠生物钟基因（包括Dec 1和Dec 2）昼夜节律表达的影响。禁食2 d后，Dec 1 mRNA表达水平下降，Per 1 mRNA表达水平显著升高，肝脏和心脏Per 2 mRNA表达水平仅在禁食42-46 h后下降; Dec 2和Bmal 1的表达谱分别仅在心脏和肝脏中改变，而Rev-erb α mRNA水平没有显著变化。饥饿36小时后重新喂养消除了，至少部分地，禁食的效果在几个小时内对Dec 1，Dec 2，Per 1，Per 2和Bmal 1，限制喂养改变了包括Dec 1和Dec 2在内的所有检查的时钟基因的表达谱的相位。这些结果表明，短期禁食和再喂食在不同程度上调节了外周组织中时钟基因的昼夜节律，并表明Dec 1，Per 1和其他一些时钟基因的表达与这些组织的代谢活动密切相关。
To elucidate the food-entrainable oscillatory mechanism of peripheral clock systems, we examined the effect of fasting on circadian expression of clock genes including Dec1 and Dec2 in mice. Withholding of food for 2 days had these effects: the expression level of Dec1 mRNA decreased in all tissues examined, although Per1 mRNA level markedly increased; Per2 expression was reduced in the liver and heart only 42-46 h after the start of fasting; and expression profiles of Dec2 and Bmal1 were altered only in the heart and in the liver, respectively, whereas Rev-erb alpha mRNA levels did not change significantly. Re-feeding after 36-h starvation erased, at least in part, the effect of fasting on Dec1, Dec2, Per1, Per2, and Bmal1 within several hours, and restriction feeding shifted the phase of expression profiles of all examined clock genes including Dec1 and Dec2. These findings indicate that short-term fasting and re-feeding modulate the circadian rhythms of clock genes to different extents in peripheral tissues, and suggest that the expression of Dec1, Per1, and some other clock genes was closely linked with the metabolic activity of these tissues.