The mRNA-like noncoding RNA Gomafu constitutes a novel nuclear domain in a subset of neurons
The mRNA-like noncoding RNA Gomafu constitutes a novel nuclear domain in a subset of neurons
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DOI：
10.1242/jcs.009357
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发表时间：
2007-08-01
影响因子：
4
通讯作者：
Nakagawa, Shinichi
中科院分区：
文献类型：
--
作者：
Sone, Masamitsu;Hayashi, Tetsutaro;Nakagawa, Shinichi
最近的转录组分析表明，哺乳动物基因组的大量非编码区实际上被转录成RNA。我们对这些非编码RNA的分子特征的理解还远未完成。我们已经确定了一种新的mRNA样非编码基因，命名为Gomafu，它在小鼠神经系统的一组不同的神经元中表达。有趣的是，剪接的成熟Gomafu RNA定位于细胞核，尽管它的mRNA样特征，这通常作为有效的输出信号到细胞质。在细胞核内，Gomafu RNA被检测为许多不与已知的核结构域标记共定位的斑点。Gomafu RNA极不溶，在核基质制备后保持完整。异核体分析表明，Gomafu RNA在转录后不穿梭于细胞核和细胞质之间，而是保留在细胞核中。我们认为Gomafu RNA代表了一个新的mRNA样非编码RNA家族，它构成了核基质的细胞类型特异性成分。
Recent transcriptome analyses have revealed that a large body of noncoding regions of mammalian genomes are actually transcribed into RNAs. Our understanding of the molecular features of these noncoding RNAs is far from complete. We have identified a novel mRNA-like noncoding gene, named Gomafu, which is expressed in a distinct set of neurons in the mouse nervous system. Interestingly, spliced mature Gomafu RNA is localized to the nucleus despite its mRNA-like characteristics, which usually act as potent export signals to the cytoplasm. Within the nucleus, Gomafu RNA is detected as numerous spots that do not colocalize with known nuclear domain markers. Gomafu RNA is extremely insoluble and remains intact after nuclear matrix preparation. Furthermore, heterokaryon assays revealed that Gomafu RNA does not shuttle between the nucleus and cytoplasm, but is retained in the nucleus after its transcription. We propose that Gomafu RNA represents a novel family of mRNA-like noncoding RNA that constitutes a cell-type-specific component of the nuclear matrix.