Differential and Interactive Effects of Scleroderma sp. and Inorganic Phosphate on Nutrient Uptake and Seedling Quality of Castanea henryi
Differential and Interactive Effects of Scleroderma sp. and Inorganic Phosphate on Nutrient Uptake and Seedling Quality of Castanea henryi
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DOI：
10.3390/agronomy12040901
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发表时间：
2022-04
期刊：
影响因子：
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通讯作者：
Ronghua Zuo;F. Zou;Shiyi Tian;J. Masabni;D. Yuan;H. Xiong
中科院分区：
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作者：
Ronghua Zuo;F. Zou;Shiyi Tian;J. Masabni;D. Yuan;H. Xiong
外生菌根真菌（ECMF）和无机磷酸盐（Pi）均能促进植物生长。然而，磷水平与菌根定植率之间的关系并不一致，而且缺乏磷水平与ECMF对养分吸收和幼苗质量的差异效应和交互效应的信息。用硬皮病菌（Scleroderma sp., +ECM）接种4周龄的甜槠（Castanea henryi’You Zhen）幼苗，比较低Pi水平（-Pi）和高Pi水平（+Pi）与未接种（-ECM）的差异。研究了菌根定植率、幼苗形态生理参数、养分吸收含量以及根际土壤酶活性和化学性质。结果表明，低磷水平下菌根定植率（84±2%）高于高磷水平（71±2.52%）；无论Pi水平如何，接种ECMF均显著提高了幼苗干重、高、径和幼苗质量指数（SQI）。与-ECM-Pi处理相比，+ECM+Pi和+ECM-Pi处理分别显著提高了282.76%和232.76%的SQI。施用Pi对SQI无显著影响。ECMF接种显著增加了根、茎和叶对氮、磷和钾的吸收，而施用Pi只增加了根对磷的吸收。除叶片磷吸收外，ECMF和Pi水平对幼苗品质和养分吸收无显著交互作用。结果表明，ECMF在保护环境、节约资源、降低生产成本等方面是一种较好的替代磷肥的育苗生产方式。
Both ectomycorrhizal fungi (ECMF) and inorganic phosphate (Pi) can improve plant growth. However, the relationship between Pi levels and mycorrhizal colonization rate is divergent, and information on the differential and interactive effects of Pi levels and ECMF on nutrient uptake and seedling quality is lacking. We conducted a study on 4-week-old Castanea henryi ‘You Zhen’ seedlings by inoculating with Scleroderma sp. (+ECM) to compare with uninoculated (-ECM), under low Pi level (-Pi) and high Pi level (+Pi). The mycorrhizal colonization rate, seedlings morphological and physiological parameters, nutrient uptake content, and the rhizosphere soil enzymatic activities and chemical properties were investigated. Results indicated that the mycorrhizal colonization rate at low Pi level (84 ± 2%) was higher than that at high Pi level (71 ± 2.52%). No matter the Pi level, inoculating with ECMF significantly improved seedling dry weight, height, diameter, and seedling quality index (SQI). The +ECM+Pi and +ECM-Pi treatments significantly increased SQI by 282.76% and 232.76% in comparison to the -ECM-Pi treatment, respectively. Applied Pi had no significant effect on SQI. ECMF inoculation significantly increased nitrogen (N), phosphorus (P), and potassium (K) uptake in roots, stems, and leaves, while Pi application increased the P uptake in roots only. There was no significant interaction between ECMF and Pi levels on seedling quality and nutrient uptake (except P uptake in leaf). Results indicated that ECMF is a suitable alternative to the use of phosphorus fertilizer in nursery production of C. henryi seedlings in terms of protecting the environment, saving resources, and reducing production costs.