TRANSCRIPTIONAL ACTIVATION BY WILD-TYPE BUT NOT TRANSFORMING MUTANTS OF THE P53 ANTIONCOGENE
TRANSCRIPTIONAL ACTIVATION BY WILD-TYPE BUT NOT TRANSFORMING MUTANTS OF THE P53 ANTIONCOGENE
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DOI：
10.1126/science.2144364
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发表时间：
1990-08-31
期刊：
影响因子：
56.9
通讯作者：
LOZANO, G
中科院分区：
文献类型：
--
作者：
RAYCROFT, L;WU, H;LOZANO, G
由野生型p53原癌基因编码的蛋白质已显示出抑制转化，而改变p53的某些突变成为转化能力。制备p53和GAL 4 DNA结合结构域之间的融合蛋白以将p53锚于DNA靶序列并允许测量报告质粒的转录激活。野生型p53刺激转录在这个试验中，但两个转化突变p53不能作为转录激活因子。因此，p53可以激活转录，并且转化激活突变导致p53蛋白的功能丧失。p53突变蛋白不能激活转录，这可能使它们具有转化能力。
The protein encoded by the wild-type p53 proto-oncogene has been shown to suppress transformation, whereas certain mutations that alter p53 become transformation competent. Fusion proteins between p53 and the GAL4 DNA binding domain were made to anchor p53 to a DNA target sequence and to allow measurement of transcriptional activation of a reporter plasmid. The wild-type p53 stimulated transcription in this assay, but two transforming mutations in p53 were unable to act as transcriptional activators. Therefore, p53 can activate transcription, and transformation-activating mutations result in a loss of function of the p53 protein. The inability of the p53 mutant proteins to activate transcription may enable them to be transformation competent.