An efficient micropropagation system for Tylophora indica:: an endangered, medicinally important plant
An efficient micropropagation system for Tylophora indica:: an endangered, medicinally important plant
复制标题
DOI：
10.1007/s11816-007-0025-4
复制
发表时间：
2007-08-01
影响因子：
2.4
通讯作者：
Anis, Mohammad
中科院分区：
文献类型：
--
作者：
Faisal, Mohd.;Ahmad, Naseem;Anis, Mohammad
描述了一种有效的方案，用于通过从两年生植物幼芽收集的节外植体增强腋芽增殖，快速体外繁殖濒危药用植物 Tylophora indica (Burm. f.) Merrill。生长调节剂[6-苄基腺嘌呤（BA）、激动素（Kin）噻苯隆（TDZ）、吲哚-3-乙酸（IAA）、吲哚-3-丁酸（IBA）或α-萘乙酸（NAA）]、抗坏血酸（AA）、不同浓度的Murashige和Skoog（MS）培养基以及不同pH水平对生长调节剂的生理作用 研究了体外形态发生。在补充有 2.5 μM BA、0.5 μM NAA 和 100 mg/l AA（pH 5.8）的 MS 培养基上记录了最高的芽数（8.6 +/- 0.71）和最大平均芽长度（5.2 +/- 0.31 cm）。在添加了 0.5 μM IBA 的半强度 MS 培养基上生根效果最好。体外再生的幼苗具有发育良好的芽和根，成功地在装有花园土壤的盆中种植，并在温室中生长，成活率达到90%。再生植物没有表现出任何可立即检测到的表型变异。所述方法可成功用于印度籼稻的大规模繁殖和长期体外保存。
An efficient protocol is described for the rapid in vitro multiplication of an endangered medicinal plant, Tylophora indica (Burm. f.) Merrill, via enhanced axillary bud proliferation from nodal explants collected from young shoots of a two-year-old plant. The physiological effects of growth regulators [6-benzyladenine (BA), kinetin (Kin) thidiazuron (TDZ), indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) or a-naphthalene acetic acid (NAA)], ascorbic acid (AA), different strengths of Murashige and Skoog ( MS) medium and various pH levels on in vitro morphogenesis were investigated. The highest number (8.6 +/- 0.71) of shoots and the maximum average shoot length (5.2 +/- 0.31 cm) were recorded on MS medium supplemented with 2.5 mu M BA, 0.5 mu M NAA and 100 mg/l AA at pH 5.8. Rooting was best achieved on half-strength MS medium augmented with 0.5 mu M IBA. The plantlets regenerated in vitro with well-developed shoot and roots were successfully established in pots containing garden soil and grown in a greenhouse with a 90% survival rate. The regenerated plants did not show any immediate detectable phenotypic variation. The described method can be successfully employed for large-scale multiplication and long-term in vitro conservation of T. indica.