Expression of cathepsin B and microvascular density increases with higher grade of astrocytomas
Expression of cathepsin B and microvascular density increases with higher grade of astrocytomas
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DOI：
10.1007/s11060-004-9163-5
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发表时间：
2005-01-01
影响因子：
3.9
通讯作者：
Teramoto, A
中科院分区：
文献类型：
--
作者：
Wang, MD;Tang, JJ;Teramoto, A
标本取自幕上胶质瘤边缘及正常脑组织，分为正常脑组织8例，星形细胞瘤30例，间变性星形细胞瘤25例，胶质母细胞瘤22例。免疫组化法检测组织蛋白酶B（CB）表达及微血管密度（MVD）。CB染色结果根据阳性细胞百分比进行评分，分为阴性（-）、弱（+）、中度（++）和强（+）。MVD采用Weiden改良法。8例正常脑组织CB阳性染色阴性。30例星形细胞瘤中只有9例显示阳性细胞百分比较低，染色呈淡、弥漫性胞浆模式（评分+）。35例间变性星形细胞瘤中22例呈浅阳性颗粒状染色，其中5例（+），17例（++）。而胶质母细胞瘤22例全部染色，呈颗粒状染色，2例染色强度为（++），20例染色强度为（+）。细胞外基质（ECM）、基底膜（BM）及血管内皮细胞染色阳性。沿着胶质瘤分级的增高，CB表达和MVD值均增高。提示MVD值与CB表达呈正相关。这强烈表明CB和血管生成在胶质瘤的进展中起着重要作用。
Samples were taken from supratentorial gliomas border and normal brain autopsy which were divided into four groups, these including eight cases normal brain tissues, 30 cases of astrocytomas, 25 cases of anaplastic astrocytomas and 22 cases of glioblastomas. Cathepin B (CB) expression and microvessel density (MVD) were determined with immunohistochemical studies. Staining results of CB was scored according to the percentage of positive cells, graded as negative (-), weak (+), moderate (++), and strong (+++). MVD was analyzed by Weidner's revised technique. CB positive staining was negative in eight cases of normal brain tissue. Only 9 out of 30 cases of astrocytomas showed a low percentage of positive cells that were stained in a light, diffuse cytoplasmic pattern (score +). Twenty-two out of 35 cases of anaplastic astrocytomas showed positive light, granular staining pattern, it including five samples (score +), and 17 samples (score ++). In contrast, all 22 cases of glioblastomas were stained all, and it was present in a course, granular staining pattern with an intensity of score (++) of two sample, and score (+++) of 20 samples. Positive staining tumor cells were found in extracellular matrix (ECM), basement membrane (BM), and the endothelial cells of blood vessels were also positive stained. Along with elevating glioma grade, CB expression and MVD value were both increased. Therefore, it showed MVD value was positive correlated with expression of CB. It highly suggested that CB and angiogenesis plays an important role in glioma progression.