Complement effects of the infectivity of Plasmodium gallinaceum to Aedes aegypti mosquitoes. II. Changes in sensitivity to complement-like factors during zygote development.
Complement effects of the infectivity of Plasmodium gallinaceum to Aedes aegypti mosquitoes. II. Changes in sensitivity to complement-like factors during zygote development.
复制标题
鸡疟原虫对埃及伊蚊的感染力的补体效应。
DOI：
--
复制
发表时间：
1987
影响因子：
1.3
通讯作者：
Richard Carter
中科院分区：
文献类型：
--
作者：
C. Grotendorst;Richard Carter
在转化为卵母细胞的过程中，鸡疟原虫的受精卵最初对其天然宿主鸡血清中的热不稳定因子和edta敏感因子的裂解具有抗性。配子发生后6至8小时，体外培养的受精卵失去对这些因素的抵抗力。对这些因素的体外抗性丧失反映在合子对埃及伊蚊的感染性丧失，在存在本地鸡血清的情况下。这些因子可能是鸡血清补体替代途径（APC）的组成部分。鸡血中的鸡鸡假单胞菌配子能够感染埃及伊蚊，显然是由于在摄入后3-4小时内，即在合子失去对鸡APC的抗性之前的几个小时内，血粉中的APC失活。除鸡血清中的热不稳定因子（APC）外，受精卵对蚊血粉中的其他因子均有短暂的敏感性。这些因子不会因事先加热血粉中的鸡血清而被破坏，因此不能作为补体成分。这些因子的抗寄生作用通过在血中添加EDTA而被中和，这可能是由于蚊子中肠中存在EDTA敏感的金属蛋白酶。
During transformation into ookinetes, the zygotes of Plasmodium gallinaceum are initially resistant to lysis by heat-labile and EDTA-sensitive factors in the serum of their natural host, the chicken. Between 6 and 8 hr postgametogenesis, zygotes cultured in vitro lose their resistance to these factors. Loss of resistance to these factors in vitro is reflected by loss of infectivity of the zygotes to Aedes aegypti mosquitoes in the presence of native chicken serum. These factors are probably components of the alternative pathway of complement (APC) of chicken serum. Gametocytes of P. gallinaceum in chicken blood are able to infect A. aegypti mosquitoes apparently due to inactivation of the APC in a blood meal within 3-4 hr after ingestion, i.e., several hours before the zygotes lose their resistance to chicken APC. In addition to the heat-labile factors (APC) in chicken serum, the zygotes are transiently sensitive to other factor(s) in the mosquito blood meal. These factor(s) are not destroyed by prior heating of the chicken serum given in a blood meal and therefore cannot be complement components. The antiparasitic effects of the factors are neutralized by addition of EDTA to the blood meal and could be due to an EDTA-sensitive metalloprotease present in the mosquito midgut.