Phenol and cresol mixture degradation by the yeast Trichosporon cutaneum
Phenol and cresol mixture degradation by the yeast Trichosporon cutaneum
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DOI：
10.1007/s10295-008-0410-1
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发表时间：
2008-11-01
影响因子：
3.4
通讯作者：
Krastanov, A.
中科院分区：
文献类型：
--
作者：
Alexieva, Z.;Gerginova, M.;Krastanov, A.
大多数工业废物中含有不同的有机混合物，因此研究复合基质的微生物破坏具有重要意义。微生物从受污染的环境中去除有害化学物质的能力很大程度上取决于其他碳和能源底物的存在。研究了苯酚和甲基取代酚(邻甲酚、间甲酚、对甲酚)混合物对皮肤毛孢菌生长行为和降解能力的影响。用高效液相色谱法对无细胞培养上清液进行分析。结果表明，邻甲酚、间甲酚和对甲酚的存在并没有阻止苯酚的完全同化，但对苯酚的降解动力学有显著的延缓作用。考察了苯酚和对甲酚的相互影响。我们在霍尔丹动力学的基础上建立了动力学模型，该模型使用单底物实验的模型参数来预测双底物混合实验的结果。估算了苯酚对对甲酚生物降解的影响程度，反之亦然。相互作用参数的定量估计对于促进单一或混合培养在危险化合物的生物处理中的应用至关重要。
Most industrial wastes contain different organic mixtures, making important the investigation on the microbial destruction of composite substrates. The capability of microbes to remove harmful chemicals from polluted environments strongly depends on the presence of other carbon and energy substrates. The effect of mixtures of phenol- and methyl-substituted phenols (o-, m-, p-cresol) on the growth behaviour and degradation capacity of Trichosporon cutaneum strain was investigated. The cell-free supernatants were analysed by HPLC. It was established that the presence of o-, m- and p- cresol has not prevented complete phenol assimilation but had significant delaying effect on the phenol degradation dynamics. The mutual influence of phenol and p-cresol was investigated. We developed the kinetic model on the basis of Haldane kinetics, which used model parameters from single-substrate experiments to predict the outcome of the two-substrate mixture experiment. The interaction coefficients indicating the degree to which phenol affects the biodegradation of p-cresol and vice versa were estimated. Quantitative estimation of interaction parameters is essential to facilitate the application of single or mixed cultures to the bio-treatment of hazardous compounds.