Search for a Novel Allergen in Hen's Egg Allergy Using an IgE Immunoblotting Assay.
Search for a Novel Allergen in Hen's Egg Allergy Using an IgE Immunoblotting Assay.
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使用 IgE 免疫印迹分析寻找鸡蛋过敏的新型过敏原。
DOI：
10.1159/000488144
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
Shimojo N.
中科院分区：
文献类型：
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作者：
Sogawa K;Takahashi Y;Shibata Y;Satoh M;Kodera Y;Nomura F;Tanaka T;Sato H;Yamaide F;Nakano T;Iwahashi K;Sugita-Konishi Y;Shimada A;Shimojo N.
背景食物过敏是一个严重的健康问题，影响着大约4%的儿童，对生活质量有很大影响。鸡蛋过敏常见于婴儿。因此，他们中的一些人不得不将鸡蛋从日常饮食中排除，以避免过敏症状。母鸡的蛋由两个可溶的部分组成；一个是蛋清，被认为是过敏的主要来源，另一个是蛋黄，据估计是一种矿物质来源。α-Livetin(Gald 5)和YGP42(Gald 6)是目前已知的两种蛋黄过敏原。采用SDS-PAGE、IgE免疫印迹和斑点印迹等方法对蛋黄进行免疫印迹。经LC-MS/MS鉴定，33 kDa条带为卵黄糖蛋白(YGP40)，斑点印迹法检测到21例(47%)有YGP40。结论YGP40是一种新的蛋黄变应原，YGP40的4个位点为线性表位。
BackgroundFood allergy is a serious health issue affecting roughly 4% of children, with a substantial effect on quality of life. Chicken egg allergy is frequently observed in infants. Therefore, some of them have to exclude hen’s eggs from their daily diet to avoid allergenic symptoms. Hen’s egg is composed of 2 soluble parts; one is egg white, which has been characterized as the major source of allergenicity, while the other is egg yolk, which is estimated as a miner source. Only 2 allergens from egg yolk, α-livetin (Gal d 5) and YGP42 (Gal d 6), have been described to date.MethodsSera from 53 patients allergic to hen’s eggs and 2 patients allergic to sesame were obtained from the Department of Pediatrics, Chiba University Hospital. The study was performed using SDS-PAGE, IgE immunoblotting, and dot blotting.ResultsSeven bands of egg yolk were detected by IgE immunoblotting. Out of these bands, a possible new allergen was further characterized by LC-MS/MS. The 33-kDa band was identified as yolk glycoprotein (YGP40) by LC-MS/MS. A total of 21 of the 53 patients (47%) had YGP40 detected by dot blotting.ConclusionsWe identified YGP40 as a new hen’s egg yolk allergen and detected 4 sites of YGP40 as linear epitopes.