Specific Detection of Subgroups I and II of Cucumber Mosaic Virus RNA with Digoxigenin-Labeled Synthetic Oligo-Deoxyribonucleotide Probes
Specific Detection of Subgroups I and II of Cucumber Mosaic Virus RNA with Digoxigenin-Labeled Synthetic Oligo-Deoxyribonucleotide Probes
复制标题
用地高辛标记的合成寡脱氧核糖核苷酸探针特异性检测黄瓜花叶病毒 RNA 亚型 I 和 II
DOI：
10.3186/jjphytopath.65.42
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
M. Takeshita
中科院分区：
文献类型：
--
作者：
Y. Takanami;T. Uchiba;T. Shigemi;K. Kikuhara;M. Takeshita
黄瓜花叶病毒 (CMV) 的基因组由三个正义单链 RNA 分子组成，按分子量递减顺序命名为 RNA1、RNA2 和 RNA3，根据血清学特性和核苷酸序列差异分为 I 亚组和 II 亚组10)。为了防止巨细胞病毒对农作物生产造成巨大损害，人们已经做出了许多努力。例如，成功地采用减毒 CMV 预接种植物来避免毒力更强的菌株的再次感染17)。许多研究还描述了在表达CMV外壳蛋白、非结构蛋白或卫星RNA(2)基因的转基因植物中建立CMV抗性。这些策略的原理基于植物病毒的•g交叉保护•h。我们现在正在使用各种 CMV 毒株研究该现象的分子基础。在这项研究过程中，我们需要开发能够区分子合成的方法
Cucumber mosaic cucumovirus (CMV) has a genome consisting of three plus-sense, single-stranded RNA molecules, designated RNA1, RNA2 and RNA3 in decreasing order of molecular weight, and is divided into subgroups I and II based on serological properties and differences in nucleotide sequence10). Many efforts have been made to prevent the enormous damage to agricultural crop production caused by CMV. For example, preinoculation of plants with attenuated CMV was successfully adopted to escape reinfection with more virulent strains17). A number of studies also describe the establishment of CMV resistance in transgenic plants which express the gene of CMV coat protein, non-structural protein or satellite RNA2). The principle of these strategies is based on •gcross-protection•h of plant viruses. We are now investigating the molecular basis of the phenomenon using various CMV strains. In the course of this study, we need to develop methods that can discriminate the syntheses of sub-