Magnetically labeled neural progenitor cells, which are localized by magnetic force, promote axon growth in organotypic Cocultures
Magnetically labeled neural progenitor cells, which are localized by magnetic force, promote axon growth in organotypic Cocultures
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DOI：
10.1097/brs.0b013e318154c651
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发表时间：
2007-10-01
期刊：
影响因子：
3
通讯作者：
Ochi, Mitsuo
中科院分区：
文献类型：
--
作者：
Hamasaki, Takahiko;Tanaka, Nobuhiro;Ochi, Mitsuo
研究设计。进行了体外大鼠动物实验。为了评估不同浓度的神经祖细胞（NPCs）和磁定位标记的NPCs中皮质脊髓轴突的生长潜力，定量使用我们的原始器官型共培养系统。背景资料摘要。脊髓损伤的npc移植被认为是一种可能的未来治疗方法。这不仅对阐明NPCs在脊髓损伤中的作用机制具有重要意义，而且对开发一种有效的临床应用的细胞递送系统具有重要意义。为了开发更有效、高效、微创的细胞递送系统，我们建立了一种新的磁性靶向系统。方法。用活化磁珠和单个npc悬浮磁性标记的npc，并将其与体外未标记的npc的特性进行比较。我们将1亩L培养基中不同浓度的102、103、104、105和106种npc移植到共培养模型中。然后将104个标记的npc用或不带磁铁移植到共培养物中。与未标记的npc相比，磁性标记的npc在轴突生长方面具有相似的潜力，因此磁性标记的npc几乎没有毒性作用。无论在体外定位还是分散，差异电位都没有改变。皮质脊髓轴突的生长与移植的NPC数量一致，这与器官型共培养周围的NPC数量一致。有磁铁的局部标记npc比没有磁铁的分散标记npc更能促进轴突的生长，因此磁性局部标记npc在轴突生长方面表现出更高的潜力。磁性标记的npc通过磁力定位，可以促进该有机型共培养系统的轴突生长。关键词：神经祖细胞，磁珠，细胞传递系统，轴突生长，共培养，脊髓损伤
Study Design. An in vitro, rat animal study was conducted.Objective. To assess the corticospinal axon growth potential in varying concentrations of neural progenitor cells (NPCs) and in magnetically localized labeled NPCs, quantitatively using our original organotypic coculture system.Summary of Background Data. Transplantation of NPCs for spinal cord injury has been anticipated as a possible future treatment. It is important not only to illuminate the mechanism of NPCs for spinal cord injury, but also to develop an effective cell delivery system for clinical use. In order to develop more effective, efficient, and minimally invasive cell delivery systems, we established a new system using magnetic targeting. Methods. Magnetically labeled NPCs were suspended with activated magnetic beads and individual NPCs, and were compared the characterization to nonlabeled NPCs in vitro. We transplanted varying concentrations of 102, 103, 104, 105, and 106 NPCs in 1 mu L medium to coculture models. Then the 104 labeled NPCs were transplanted with or without magnet to the cocultures.Results. Magnetically labeled NPCs had similar potential in axon growth compared with nonlabeled NPCs, so there were few toxic effects of magnetically labeling NPCs. The differential potentials were not changed whether they were localized or scattered in vitro. Corticospinal axon growth was promoted in accordance with the transplanted NPC numbers around the organotypic coculture. Localized labeled NPCs with a magnet promoted axon growth much more than scattered labeled NPCs without a magnet, so magnetically localized labeled NPCs expressed higher potential in axon growth.Conclusion. Magnetically labeled NPCs, which were localized by magnetic force, could promote axon growth in this organotypic coculture system. Key words: neural progenitor cell, magnetic beads, cell delivery system, axon growth, coculture, spinal cord injury.