Proteomics reveal a concerted upregulation of methionine metabolic pathway enzymes, and downregulation of carbonic anhydrase-III, in betaine supplemented ethanol-fed rats.
Proteomics reveal a concerted upregulation of methionine metabolic pathway enzymes, and downregulation of carbonic anhydrase-III, in betaine supplemented ethanol-fed rats.
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DOI：
10.1016/j.bbrc.2009.02.082
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发表时间：
2009-04-17
影响因子：
3.1
通讯作者：
Carter, Wayne G.
中科院分区：
文献类型：
--
作者：
Kharbanda, Kusum K.;Vigneswara, Vasanthy;McVicker, Benita L.;Newlaczyl, Anna U.;Bailey, Kevin;Tuma, Dean;Ray, David E.;Carter, Wayne G.
关键词：
我们采用蛋白质组学分析策略来研究乙醇和甜菜碱饮食补充对主要肝脏蛋白质水平变化的影响。雄性Wistar大鼠喂养对照，乙醇或甜菜碱补充饮食4周。肝脏被删除和肝脏胞质蛋白解决了一维和二维分离技术。甜菜碱同型半胱氨酸甲基转移酶-1，甲硫氨酸腺苷转移酶-1和甘氨酸N-甲基转移酶的显著上调是在喂食甜菜碱补充乙醇饮食的大鼠肝脏中观察到的最明显的蛋白质变化。我们推测，这些蛋氨酸代谢途径酶的协同上调是甜菜碱恢复肝脏S-腺苷蛋氨酸与S-腺苷高半胱氨酸的正常代谢比的保护机制。乙醇还诱导碳酸酐酶-III蛋白水平的显着下调，甜菜碱补充不能恢复。碳酸酐酶-III可以起到抵抗氧化应激的作用，因此我们假设，碳酸酐酶-III蛋白水平受到乙醇消耗的影响，有助于乙醇诱导的氧化还原应激。
We employed a proteomic profiling strategy to examine the effects of ethanol and betaine diet supplementation on major liver protein level changes. Male Wistar rats were fed control, ethanol or betaine supplemented diets for 4 weeks. Livers were removed and liver cytosolic proteins resolved by one-dimensional and two-dimensional separation techniques. Significant upregulation of betaine homocysteine methyltransferase-1, methionine adenosyl transferase-1, and glycine N-methyltransferase were the most visually prominent protein changes observed in livers of rats fed the betaine supplemented ethanol diet. We hypothesise that this concerted upregulation of these methionine metabolic pathway enzymes is the protective mechanism by which betaine restores a normal metabolic ratio of liver S-adenosylmethionine to S-adenosylhomocysteine. Ethanol also induced significant downregulation of carbonic anhydrase-III protein levels which was not restored by betaine supplementation. Carbonic anhydrase-III can function to resist oxidative stress, and we therefore hypothesise that carbonic anhydrase-III protein levels compromised by ethanol consumption, contribute to ethanol-induced redox stress.
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影响因子：
3.9
作者：
LII, CK;CHAI, YC;HENDRICH, S
通讯作者：
HENDRICH, S
DOI：
10.1152/ajpgi.00044.2006
发表时间：
2006-11-01
影响因子：
4.5
作者：
Bailey, Shannon M.;Robinson, Gloria;Darley-Usmar, Victor
通讯作者：
Darley-Usmar, Victor
影响因子：
4.8
作者：
Vigneswara, Vasanthy;Lowenson, Jonathan D.;Carter, Wayne G.
通讯作者：
Carter, Wayne G.
影响因子：
3.7
作者：
Mallis, RJ;Hamann, MJ;Thornas, JA
通讯作者：
Thornas, JA
影响因子：
5.8
作者：
Kharbanda, KK;Rogers, DD;Tuma, DJ
通讯作者：
Tuma, DJ