Optical imaging of reporter gene expression using a positron-emission-tomography probe
Optical imaging of reporter gene expression using a positron-emission-tomography probe
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DOI：
10.1117/1.3514659
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发表时间：
2010-11-01
影响因子：
3.5
通讯作者：
Cheng, Zhen
中科院分区：
文献类型：
--
作者：
Liu, Hongguang;Ren, Gang;Cheng, Zhen
报告基因/报告探针技术是分子影像学中最重要的技术之一。最近，许多报告基因/报告探针系统已经与不同的成像模式如正电子发射断层扫描（PET）和光学成像（01）结合。最近发现，01技术可用于在体外和活体中监测放射性示踪剂。在这项研究中，我们进一步证明了一个报告基因/核报告探针系统[单纯疱疹病毒1型胸苷激酶（HSV 1-tk）和9-（4-F-18-氟-3-羟甲基]丁基）鸟嘌呤（[F-18] FHBG）] 1可以成功地成像由01在体外和体内。01与放射性报告探针的结合将促进和拓宽报告基因/报告探针技术在医学研究中的应用。(C)2010年，美国光学仪器工程师学会（Society of Photo-Optical Instrumentation Engineers）[DOI 10.1117/1.3514659]
Reporter gene/reporter probe technology is one of the most important techniques in molecular imaging. Lately, many reporter gene/reporter probe systems have been coupled to different imaging modalities such as positron emission tomography (PET) and optical imaging (01). It has been recently found that 01 techniques could be used to monitor radioactive tracers in vitro and in living subjects. In this study, we further demonstrate that a reporter gene/nuclear reporter probe system [herpes simplex virus type-1 thymidine kinase (HSV1-tk) and 9-(4-F-18-fluoro3-hydroxymethyl] butyl) guanine ([F-18] FHBG)1 could be successfully imaged by 01 in vitro and in vivo. 01 with radioactive reporter probes will facilitate and broaden the applications of reporter gene/reporter probe techniques in medical research. (C) 2010 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3514659]